胃肠宁对利血平所致大鼠脾虚模型的影响  

Influence of Weichangning,a Chinese medicine decoction,on rats with spleen deficiency syndrome induced by reserpine

作　　者：郭延生[1] 吴玉泓[2] 华永丽[1] 杨洪盛[1] 魏彦明[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃中医学院基础部,兰州730000

出　　处：《中国实验动物学报》2011年第3期242-245,282,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金："十一五"国家科技支撑计划项目(编号:2008BADB4B01-4)

摘　　要：目的观察胃肠宁对大鼠脾虚证模型的影响。方法 36只大鼠随机分为正常对照组、脾虚模型组、四君子汤组和胃肠宁组,每组9只。采用利血平复制大鼠脾虚模型,连续灌胃14 d后,处死大鼠,分离肝、十二指肠和血清,测定各组大鼠血浆LDH、AKP、AchE、CK和肝组织匀浆中SOD、MDA、GSH-Px、NOS含量变化,并观察各组大鼠肝和十二指肠病理变化。结果与正常对照组相比,脾虚模型组大鼠LDH、AchE、CK、SOD、GSH-Px、NOS含量明显降低,而AKP、MDA含量明显升高;与脾虚模型组相比,胃肠宁组LDH、AchE、CK、SOD、GSH-Px、NOS含量显著升高,而AKP、MDA含量显著降低;脾虚模型组大鼠肝组织发生广泛性颗粒变性和空泡变性;十二指肠上皮及绒毛明显脱落,大量炎性细胞浸润,杯状细胞数量明显减少,胃肠宁组与脾虚组相比,肝和十二指肠病理变化明显减轻。结论胃肠宁对利血平所致大鼠脾虚具有较好的治疗作用。Objective To study the influence of weichangning,a Chinese medicine decoction,on rat models of experimantal spleen deficiency syndrome.Methods Thirt-six male Wistar rats were randomly divided into 4 groups： normal control group,spleen deficiency syndrome model group,Sijunzitang（another Chinese medicine decoction） group and Weichangning group,9 rats in each group.The rat models of spleen deficiency syndrome were induced by resperpine.The plasma concentrations of LDH,AKP,AchE and CK and the contents of SOD,MDA,GSH-Px and NOS in liver tissue were measured.The pathological changes of liver and duodenum were observed by histo pathology.Results Compared with the normal control group,the concentrations of LDH,AchE,CK,SOD,GSH-Px and NOS were significantly decreased,and AKP and MDA levels increased in the spleen deficiency syndrome model group.Compared with the spleen deficiency syndrome model group,the concentrations of LDH,AchE,CK,SOD,GSH-Px and NOS were significantly increased,and AKP and MDA levels decreased in the Weichangning groups.In the rats of spleen deficiency syndrome model group,the liver showed granular degeneration and vacuolation,and the duodenal epithelial cells showed a decreased amount of villi and goblet cells,and infiltration of inflammatory cells.The pathological changes of liver and duodenum in the Weichangning group were obviously slighter than that in the spleen deficiency syndrome model group.ConclusionWeichanning has an apparent therapeutic effect on the rat models of spleen deficiency syndrome.

关 键 词：胃肠宁 利血平 大鼠 脾虚模型 

分 类 号：Q95-33[生物学—动物学] R-285[医药卫生]