胃癌组织Wnt5a和MMP14表达及其临床意义的探讨  

Expressions and clinical significances of Wnt5a and MMP14 in gastric cancer

作　　者：江州华[1] 俞继卫[1] 吴生华[1] 李小强[2] 吴巨钢[1] 姜波健[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普外科,上海201900 [2]上海交通大学医学院附属第三人民医院病理科,上海201900

出　　处：《中华肿瘤防治杂志》2011年第12期940-945,共6页Chinese Journal of Cancer Prevention and Treatment

基　　金：上海交通大学医学院科研项目(2007XJ0322009XJ21037)

摘　　要：目的:研究Wnt5a和MMP14在胃癌组织中的表达,分析其与临床病理特征及预后的关系,并探索两者间表达的相关性。方法:Real-timePCR及免疫组化染色法检测胃癌原发灶和癌旁正常胃组织中Wnt5a和MMP14的表达,单因素和多因素分析其表达与临床病理特征和预后的关系。结果:胃癌原发灶中Wnt5a和MMP14在转录和翻译水平上的表达均明显高于癌旁正常胃组织中的表达(P<0.05),并且两者在胃癌原发灶中的表达呈正相关。此外,Wnt5a阳性表达组的肿瘤直径更大、位置更高、浸润深度更深、区域淋巴结转移数更多、TNM分期更晚及淋巴结转移率更高,存在淋巴管和血管浸润,P<0.05。MMP14阳性表达组与淋巴管浸润有关,但与血管浸润无关,且与浸润深度更深、区域淋巴结转移数更多、TNM分期更晚及淋巴结转移率更高相关,P<0.05。单因素分析发现Wnt5a阳性表达组的预后更差,MMP14表达与预后无关。但多因素Cox逐步回归模型分析仅发现浸润深度和区域淋巴结转移数是胃癌独立的预后因素。结论:Wnt5a和MMP14的表达在胃癌组织中呈正相关,可促进胃癌的侵袭和转移。此外,Wnt5a阳性表达还提示预后更差。OBJECTIVE： To investigate the expressions of Wnt5a and MMP14 in gastric cancer, as well as the relationship among Wnt5a, MMP14, clinicopathological characteristics and the influences of them on the prognosis. METHODS： Expressions of WntSa and MMP14 in primary lesions of gastric cancer and the adjacent normal gastric tissues were detected by Real-time PCR and immunohistochemical staining, Univariate and multivariate analysis were used to analyze the relationship among them, clinicopathological characteristics and the influences of them on the prognosis. RESULTS： Expressions of Wnt5a and MMP14 in the level of transcription and translation in primary lesions of gastric cancer are significantly higher than that in adjacent normal gas tric tissues respectively, P〈0.05, and there are positive relation be tween them in primary lesions of gastric cancer. In addition, positive expression of WntSa was found to associate with larger tumor diameter, higher location, deeper depth of invasion, more number of regional lymph node metastasis, later TNM stage and higher rate of lymph node metastasis, lymphatic infiltration and vascular infiltration, P〈 0.05. The expression of MMP14 associated with lymphatic infiltration, but not with vascular infiltration. Higher expression of MMP14 was correlated with deeper tumor invasion, more number of regional lymph node metastasis, later TNM stage and higher rate of lymph node metastasis, P〈0.05. Log rank test found that poor prognosis was associated with positive expression of Wnt5a. However, multivariate＇s Cox regression model analysis revealed only the depth of invasion and regional lymph node metastasis were the independent prognostic factors of gastric cancer. CONCLUSIONS： Higher expression of WNTSa and MMP14 seems to promote invasion and metastasis of gastric cancer, and there are positive relation between their expressions. Furthermore, WntSa expression also suggests there may be even worse prognosis.

关 键 词：胃肿瘤 侵袭 转移 WNT5A MMP14/MT1-MMP 

分 类 号：R735.2[医药卫生—肿瘤]