雌孕激素及米非司酮对人子宫内膜癌细胞孕激素受体亚型调控的研究  

Regulation of estrogen,progesterone and mifepristone on the expression of two human progesterone receptor isoforms in endometrial carcinoma cells

作　　者：李大鹏[1,2] 盛修贵[1,2] 魏萍[1,2] 魏玲[1,3] 宋宝[1,3] 

机构地区：[1]山东省医学科学院 [2]山东省肿瘤医院妇瘤科,山东济南250117 [3]山东省肿瘤医院中心实验室,山东济南250117

出　　处：《中华肿瘤防治杂志》2011年第11期856-858,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省自然科学基金(Y2007C146)

摘　　要：目的:通过体外实验探讨雌激素、孕激素及米非司酮对人子宫内膜癌细胞孕激素受体亚型表达的调节。方法:体外培养表达不同孕激素受体亚型的人子宫内膜癌细胞HECCA-A(PRA+)、HECCA-B(PRB+)及HECCA-AB(PRA+和PRB+),分别加入雌激素、孕激素和米非司酮,作用24、48h后采用蛋白质印迹法测定各组细胞中2种孕激素受体亚型蛋白的表达。结果:雌激素作用48 h后,HECCA-A、HECCA-AB细胞的PRA表达水平明显升高,PRA/PRB比值升高,P<0.05;孕激素及米非司酮作用48 h后,HECCA-B、HECCA-AB细胞的PRB表达水平明显降低,PRA/PRB比值升高,P<0.05。结论:雌激素上调子宫内膜癌细胞PRA的蛋白表达水平,孕激素及米非司酮下调PRB的蛋白表达水平。OBJECTIVE： To investigate the regulatory effects of strogen, progesterone and mifepristone on the expression of two human progesterone receptor isoforms in endometrial carcinoma cells in vitro. METHODS： Three endometrial carcinoma cell lines were cultured in vitro ： HECCA-A （PRA ＋ ）, HECCA-B （PRB ＋） and HECCA-AB （PRA~ ,PRB＋）. The cells were incubated with estrogen, progesterone and mifepristone for 24 hours and 48 hours individually. The expressions of two progesterone receptor isoforms were detected by Western Blot. RESULTS： When stimu- lated by estrogen for 48 hours, the expression of PRA and the rati o of PRA/PRB in HECCA-A and HECC^AB ceils raised significandy （P〈0.05）, while after being stimulated by progesterone or mifepristone, the expression of PRB in HECCA B and HECCA AB cells decreased, and the ratio of PRA/PRB raised significiantly （P〈0.05）. CONCLUSION： Estrogen can up-regulate the expres sion of PRA at protein level, progesterone or mifepristone can decrease the protein expression of PRB.

关 键 词：子宫内膜肿瘤 受体 孕激素 雌激素类 米非司酮 

分 类 号：R737.33[医药卫生—肿瘤]