肿瘤坏死因子α和白介素6及核因子κB在酒精性肝病患者血清中的表达及意义  

The Expression and Significance of TNF-α,IL-6,and NF-κB in the Serum of Patients with Alcoholic Liver Disease

作　　者：吕海燕[1] 王沁[1] 张曦[1] 

机构地区：[1]兰州大学第一医院消化科,甘肃省兰州市730000

出　　处：《中国全科医学》2011年第21期2405-2407,共3页Chinese General Practice

摘　　要：目的探讨肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、核因子κB(NF-κB)在酒精性肝病(ALD)患者血清中的表达及意义,研究细胞凋亡在ALD中的作用。方法采用酶联免疫吸附试验(ELISA)法检测54例ALD患者(病例组)和31例健康体检者(正常对照组)血清中的TNF-α、IL-6、NF-κB水平,推测细胞因子及凋亡在ALD发病中的作用;采用全自动生化仪检测两组血清γ谷氨酰转移酶(GGT)、天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)水平。结果 (1)ALD患者血清TNF-α、IL-6、NF-κB、GGT、AST、ALT水平均显著高于正常对照组,差异有统计学意义(P<0.05);(2)血清TNF-α、IL-6、GGT水平之间呈正相关(P<0.05)。结论 TNF-α、IL-6和NF-κB作为炎症因子和核转录因子在ALD的发生发展中起到重要的作用,联合检测对于判断ALD患者肝脏的炎症反应和肝细胞凋亡有一定的参考价值。Objective To explore the expression and significance of TNF-α,IL-6,NF-κB in alcoholic liver disease（ALD） patients and the role of apoptosis in ALD.Methods Serum levels of TNF-α,IL-6,and NF-κB of 54 ALD patients and 31 healthy subjects were analyzed using enzyme-linked immunosorbent assay（ELISA） method,with the role these cytokines played in the pathogenesis of ALD.Levels of GGT,aspartate aminotransferase（AST）,and alanine aminotransferase（ALT）were measured by an automatic biochemical analyzer.Results（1）Serum levels of TNF-α,IL-6,NF-κB,GGT,AST,and ALT were significantly higher in the ALD group than in the control group（P0.05）;（2） Positive correlations existed among serum levels of TNF-α,IL-6,and GGT（P0.05）.Conclusion As inflammatory factors and nuclear transcription factors,TNF-α,IL-6,NF-κB play important roles in the pathogenesis of ALD and combination detection could provide certain references in reading the liver inflammatory responses and hepatocyte apoptosis among ALD patients.

关 键 词：酒精性肝病 肿瘤坏死因子Α 白介素6 NF-ΚB 细胞凋亡 

分 类 号：R575[医药卫生—消化系统]