宫颈癌与癌前病变中微小染色体维持蛋白7表达及与HPV16感染的关系  

The expression of MCM7 in CIN and cervical cancer and the relation with the infection of HPV16

作　　者：李义飞[1] 程大丽[2] 王惠兰[1] 文顺金[1] 贺英[1] 国雪[1] 

机构地区：[1]河北医科大学第二医院妇产科,河北石家庄050000 [2]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《中国实用妇科与产科杂志》2011年第7期545-548,共4页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：河北省卫生厅重点跟踪项目GL200308

摘　　要：目的探讨宫颈病变组织中微小染色体维持蛋白7(MCM7)mRNA的表达及与HPV-16E7 mRNA的关系。方法采用半定量RT-PCR的方法检测2004年9月至2005年12月河北医科大学第二、第四医院102例宫颈组织中MCM7 mRNA和HPV-16E7 mRNA的表达。结果 (1)HPV-16E7 mRNA的表达水平在CINⅡ～Ⅲ及宫颈鳞癌明显高于慢性宫颈炎和CINⅠ(P<0.05);(2)MCM7 mRNA的表达水平在CINⅡ～Ⅲ及宫颈鳞癌明显高于慢性宫颈炎和CINⅠ,宫颈鳞癌明显高于CINⅡ～Ⅲ(P<0.05);(3)在CIN中,MCM7 mRNA在HPV16阳性组表达明显高于阴性组(P<0.01),在宫颈浸润癌中MCM7与HPV-16E7表达水平呈正相关(P<0.05);(4)在宫颈浸润癌中,MCM7 mRNA的表达随肿瘤分化程度增加而减少(P<0.05),而与年龄、临床分期无关。结论 HPV-16E7 mRNA的表达水平与宫颈病变的进展相关;MCM7 mRNA的表达与宫颈癌的发生、发展及与HPV-16感染密切相关。MCM7有望成为宫颈癌前病变筛查、监测生物学指标。Objective To investigate the expression of MCM7 mRNA and the relationship with the infection of HPV16 E7 mRNA in different cervical lesion. Method The quantities of MCM7 mRNA and HPV16 E7 mRNA were detected by RT-PCR in 102 patients. Result （ 1 ） The expression of HPV16 E7 mRNA in CIN Ⅱ- Ⅲ and cervical cancer was significant higher than that in chronic cervicitis or the group of CIN Ⅰ （P 〈 0. 05 ）. （2） The expression of MCM7 mRNA was higher in CIN Ⅱ- Ⅲ and cervical cancer than that in chronic cervicitis or the group of CIN Ⅰ . there was also significant difference between the group of CIN Ⅱ- Ⅲ and the group of cervical cancer （P 〈 0. 05）. （3） The expression of MCM7 mRNA was higher in HPV16 E7 positive than that in negative group （ P 〈 0. 01 ） ; The expression of MCM7, HPV16 E7 showed positive correlation in invasive cervical cancer （P 〈 0. 05 ）. （4）The expression of MCM7 mRNA decreased with the increasing degree of tumor differentiation （P 〈 0. 05） , while its expression showed no relation with age and clinical stage. Conclusion The expression of HPV16 E7 mRNA relates with the cervical lesion progress. The expression of MCM7 relates with the occurrence and development of cervical cancer and the infection of HPV16. MCM7 is expected to be the biomarker of screening, monitoring, evaluation in cervical precancerous lesions.

关 键 词：微小染色体维持蛋白 逆转录聚合酶链反应 宫颈癌 HPV16 E7 

分 类 号：R737.33[医药卫生—肿瘤]