超声波辅助脂肪酶催化拆分丁酸环氧丙酯  

Ultrasound-Promoted Resolution of (R,S)-Glycidyl Butyrate Catalyzed by Lipase

作　　者：王佳欣[1] 孙若溪[2] 荀二娜[1] 张爱军[1] 王任[1] 魏晓飞[1] 王磊[1] 王智[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130012 [2]吉林大学生命科学学院,长春130012

出　　处：《吉林大学学报（理学版）》2011年第4期782-786,共5页Journal of Jilin University:Science Edition

基　　金：国家自然科学基金(批准号:31070708;20803028;30870539);吉林大学基本科研业务费项目(批准号:200903099)

摘　　要：利用超声波辅助脂肪酶催化拆分丁酸环氧丙酯,考察了超声发生装置、超声处理方法、超声功率及超声温度等反应条件对酶促拆分反应的影响,筛选得到最适反应条件.结果表明,在最适反应条件下,超声作用可以使酶活提高近4.3倍,立体选择性提高5倍.The ultrasound was used to promote the resolution of glycidyl butyrate catalyzed by lipase.The effects of ultrasound source,ultrasonic processing method,power and temperature were also investigated to obtain the optimum reaction conditions.Under the optimum conditions,the enzyme activity of BSL2 in the resolution of glycidyl butyrate was enhanced by about 4.3-fold and the enantioselectivity increased by about 5.0-fold.

关 键 词：超声 脂肪酶 丁酸环氧丙酯 拆分 

分 类 号：Q814.9[生物学—生物工程]