毛细管电泳对大鼠炎症模型的代谢组学研究  

Monitoring Urine Metabolic Profiles of Rat Inflammation Model with Capillary Electrophoresis

作　　者：刘羽洁[1] 苗文娟[1] 尹海林[2] 叶利明[1,3] 陈聪[1] 张志荣[3] 

机构地区：[1]四川大学华西药学院药物分析教研室,成都610041 [2]四川大学实验动物中心,成都610041 [3]四川大学华西药学院药物靶向重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2011年第4期560-563,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的建立尿液代谢物组的毛细管电泳分析方法,了解炎症模型大鼠的尿液代谢物轮廓变化。方法基于代谢组学方法,利用毛细管区带电泳分析正常大鼠、炎症模型大鼠尿液代谢物数据,用计算机辅助相似度评价系统进行峰匹配和数据分析,结合主成分分析方法,研究炎症模型大鼠的尿液代谢轮廓。结果大鼠尿液毛细管电泳图谱经主成分分析显示炎症大鼠尿液与正常大鼠尿液代谢物表型不同,并且不受性别差异、个体差异、昼夜差异、日间差异的影响。结论基于毛细管电泳的代谢组学技术可以为炎症模型的尿液代谢物轮廓分析提供研究手段,为炎症疾病及药物代谢组学的进一步研究奠定了基础。Objective To determine the comprehensive metabolic profiling of inflammation with capillary zone electrophoresis（CZE） in rat urine.Methods CZE-based metabolomics method was used to acquire urine metabolom data,with Computer Aided Similarity Evaluation System aligning peaks and analyzing urine profiling.Principle component analysis（PCA） was performed to compare and classify the phenotypes of the urine CZE spectra.Results The PCA revealed different phenotypes of metabolites between rats with and without inflammation,which were independent from gender,individual diversity,day-night diversity and diurnal variation.Conclusion CZE-based metabolomics method could be used as a potential tool for urinary profiling for disease diagnosis and drug studies.

关 键 词：毛细管电泳 尿液代谢轮廓 炎症模型 主成分分析 

分 类 号：R-332[医药卫生]