儿童肾母细胞瘤HMGB1、RAGE及VEGF的表达及意义  

The expression and significance of HMGB1. RAGE and VEGF in Wilms ＇ tumor

作　　者：张大[1] 张国锋[1] 王家祥[1] 刘秋亮[1] 范应中[1] 张谦[1] 马威[1] 穆鑫[1] 孟庆磊[1] 

机构地区：[1]郑州大学第一附属医院小儿外科,450052

出　　处：《中华小儿外科杂志》2011年第7期534-538,共5页Chinese Journal of Pediatric Surgery

基　　金：基金项目：国家自然科学基金资助项目（编号：81071782）;卫生部项目（编号：20090111）

摘　　要：目的探讨高迁移率族蛋白-1（HMGB1）、晚期糖基化终末产物受体（RAGE）和血管内皮生长因子（VEGF）在小儿肾母细胞瘤组织中的表达及其在肿瘤血管浸润、淋巴结转移中的意义。方法收集郑州大学第一附属医院2003年1月至2010年1月＆Jg#b科手术切除经病理证实为肾母细胞瘤的石蜡标本50例、15例同期相应瘤旁组织和7例正常肾组织标本。应用免疫组织化学SP染色辅以计算机图像分析系统分析结果的方法，研究HMGB1、RAGE及VEGF在各组标本中的表达及意义。结果HMGB1、RA（记在肾母细胞瘤组织中的表达（144．46±13．55、138．18±12．26）与两者在瘤旁组织和正常肾组织中表达（107．47±5．27、103．91±4．29；100．98±4．82、100．82±3．32）相比差异均有统计学意义（P〈0．05），HMGB1在瘤旁与正常肾之间表达差异有统计学意义（P〈0．05）。VEGF在瘤体组与瘤旁组中（147．57±13．77，140．28±7．85）和在正常肾组织组中的表达（106．38±1．92）相比差异显著（P〈0．05）。三者在临床分期Ⅲ～Ⅳ期和预后不良组织型组及淋巴结转移组的肾母细胞瘤组织中的表达（148．69±12．17、147．73±6．71、163．14±7．50；157．88±4．44、155．29±3．97、169．17±4．42；152．11±7．36、151．56±5．46、163．83±7．20）显著高于临床分期I～Ⅱ期和预后良好组织型及无淋巴结转移组（P〈0．05）（139．23±5．83、133．3（）±11．23、140．85±8．87；139．52±5．22、135．39±9．71、143．94±10．39；138．61±5．59、133．00±8．75、141．18±8．95）。相关分析显示肾母细胞瘤组织中HMGB1与RAGE、VE（订的表达均成正相关（r：0．424，P=0．002；r=0．453，P=0．001），RAGE与VEGF之间无明显相关（r=0．237，P=0．101）。结论HMGB1、RAGE和ⅦGF的高表达与。肾母细胞瘤的临床分期、病理类型、淋巴结转移有关，参与了肿瘤血管浸润及Objective This study aim to assess the expressions of high mobility group box chro- mosomal proteinl （HMGB1） and receptor foradvanced glycation end products （RAGE） and vascular endothelial growth factor （VEGF） in Wilms＇tumor and their clinical significance both in the tumor blood vessel infiltrates and in the lymph node metastasis. Methods Fifty cases of Wilms＇tumor sam- ples, which had been confirmed by pathology, were collected from The First Affiliated Hospitals of Zhengzhou University from january 2003 to january 2010. And 15 cases were taken from the adjacent kidney tissues at the same time. Other 7 cases were normal kidney tissues. The expression of HMGB1, RAGE and VEGF were detected by the Immunohistochemical SP staining in each group of specimens. The expression intensity was analyzed by computer image processing and their significance in each group of specimens were studied. Results The expressions of HMGB1 and RAGE in the Wilms＇tumor tissues （144. 46 ± 13.55,138. 18 ± 12. 26） were compared with those in the adjacent kid- ney tissues and in the normal kidney tissues（ 107. 47 ± 5. 27,103. 91 ± 4. 29; 100. 98 ± 4. 82,100. 82 ± 3. 32）. The expressions of HMGB1 in the adjacent kidney tissues and in the normal kidney showed significant differences. There was a significant differences between the Wilms＇ tumor group and theadjacent kidney tissues or in the normal kidney tissues （ P 〈 0.05 ）. The expressions of VEGF proteins in Wilms＇ tumor tissues and in the adjacent kidney tissues（147. 57± 13. 77,140. 28± 7. 85） compared to those in the normal kidneytissues（106. 38 ±1.92）were remarkably different（P〈0. 05）. The expressions of HMGB1, RAGE and VEGF proteins in Wilms tumor tissues of stage III-IV and high risk histopathology and lymph node metastasis group （148. 69 ± 12. 17,147. 73± 6. 71,163. 14 ±7. 50; 157. 88 ± 4. 44,155.29 ± 3.97, 169. 17 ±4. 42,152. 11 ± 7. 36,151.56 ± 5.46,163.83 ± 7. 20） were significantly higher than those of stage I-I

关 键 词：肾母细胞瘤 高迁移率族蛋白-1 晚期糖基化终末产物受体 血管内皮生长因子 

分 类 号：R737.11[医药卫生—肿瘤]