高危HPV病毒第2代杂交捕获法检测应用于宫颈癌以及癌前病变检查  

Application of Hybrid Capture 2 for High-Risk Human Papillomavirus Testing in Cervical Cancer and Premalignant Lesion

作　　者：张玉梅[1] 曹维克[2] 

机构地区：[1]南京医科大学附属淮安第一医院妇科,江苏淮安223300 [2]南京医科大学附属淮安第一医院中心实验室,江苏淮安223300

出　　处：《武汉大学学报（医学版）》2011年第4期489-491,496,共4页Medical Journal of Wuhan University

摘　　要：目的:探讨高危人类乳头瘤病毒(HPV)第二代杂交捕获实验(hc2)检测应用于宫颈癌以及癌前病变检查中的临床价值。方法:用hc2检测其子宫颈脱落细胞,以病理诊断作为金标准对检测结果进行分析和评价,对其特异度、敏感度、准确性、预测值等检测并分析。结果:hc2法检测高危型HPV的感染状况为无明确意义的非典型细胞的改变(ASCUS)44.7%,不典型鳞状细胞不除外高度病变(ASCUS-H)7.8%,低度鳞状上皮内病变(LSIL)19.7%,高级别鳞状上皮内病变(HSIL)28.7%,诊断宫颈高度病变(≥CINⅡ)的各项值分别为66.7%,93.4%,60.0%,95.0%,其中在高病毒负荷量中,只有宫颈癌与宫颈CINⅠ之间的差异显著,P<0.01,CIN各组以及宫颈癌组与无上皮内病变(NILM)组比较有显著性差异,P<0.01,而CIN各组以及宫颈癌组间比较无显著性差异,P>0.05。结论:hc2高危型HPV病毒检测仍是目前宫颈癌及癌前病变筛查较好的方法,宫颈癌hc-2法检测HR-HPV病毒载量与CIN以及宫颈癌发生有关,但与宫颈病变的严重程度无关。Objective：To explore the clinical value of hybrid capture 2（hc2） in detection of high risk HPV in cervical cancer and pre-cancerous cervical lesions.Methods：Cervix exfoliated cells were detected with the second generation of hybrid capture hc2 method,and the diagnostic results such as specificity,sensitivity,accuracy,and pedicted value were compared with that of pathological diagnosis respectively.Results：The infection rate of HPV assayed by hc2 was 43.8% in atypical squamous cell of undetermined signification（ASCUS）,7.8% in ASCUS-H,9.7% low-grade squamous intraepithelial lesion（LSIL）,and 28.7% in high-grade squamous intraepithelial lesion（HSIL） respectively according to TBS diagnosis and classification system.For high grade-squmous intraepithelial lesion≥（CINⅡ）,the specificity,sensitivity,positive-predictive value and negative-predictive value by hc2 were 66.7%,93.4%,60.0%,and 95.0% respectively.In high virus load assay,there was significant difference between cervical cancer and CINⅠ（P0.01）,and between cervical cancer/CIN and NILM group（P0.01）,but there was no difference between CIN and cervical cancer.Conclusion：The high-risk HPV test with hc2 is still a good method in diagnosing cervical cancer and premalignant lesion.Examining the high-risk HPV viral load with hc2 is related to cervical cancer and premalignant lesions,but not related to the severity of cervical lesions.

关 键 词：HPV病毒 hc2检测 宫颈癌 癌前病变 

分 类 号：R737.33[医药卫生—肿瘤]