基质细胞衍生因子-1在氧诱导视网膜病变小鼠视网膜中的表达  

Expression of stromal cell-derived factor-1 in mouse retina with oxygen-induced retinopathy

作　　者：种泽龙[1] 韩泉洪[1] 赵堪兴[1] 

机构地区：[1]眼科医院天津市眼科学与视觉科学重点实验室天津市眼科研究所天津医科大学眼科临床学院,天津市300020

出　　处：《中华实验眼科杂志》2011年第7期625-629,共5页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景早产儿视网膜病变（ROP）的进展与多种新生血管调控因子有关，而基质细胞衍生因子·1（SDF．1）在氧诱导视网膜病变（OIR）动物模型视网膜中的表达及其作用机制尚不明确。目的对SDF-1在OIR小鼠模型视网膜中的表达进行定位和定量分析。方法应用随机数字表法将动物随机分组。将20只7日龄C57BL／6J幼鼠暴露在体积分数（75±2）％浓度的高氧状态下5d，随后在正常氧环境下5d，作为OIR组；另20只同日龄幼鼠作为正常对照组。通过免疫组织化学染色和实时荧光定量聚合酶链反应（real-timePCR）法观察视网膜的SDF-1的蛋白表达以及SDF-1mRNA的变化。结果OIR组12日龄小鼠的视网膜神经节细胞层可见SDF．1蛋白呈阳性表达；OIR组17习龄小鼠的神经节细胞层、内层视网膜的血管内皮细胞、新生血管内皮细胞可见SDF-1蛋白呈强阳性表达；正常对照组12日龄小鼠SDF-1蛋白和正常对照组17日龄小鼠SDF-1蛋白微弱表达于内层视网膜及视网膜血管附近。OIR组17日龄小鼠的SDF-1mRNA表达水平明显高于OIR组12日龄小鼠（P〈O．01）及正常对照组17日龄小鼠（P〈0．01）；OIR组12日龄小鼠SDF-1mRNA表达水平明显低于正常对照组12日龄小鼠（P〈0．05）。OIR组17日龄小鼠的SDF-1mRNA表达水平明显高于OIR组12日龄小鼠（t=8．072，P〈0．05）和正常对照组17日龄小鼠（t=10．026，P〈O．05）；OIR组12日龄小鼠SDF-1mRNA表达水平明显低于正常对照组12日龄小鼠（t=4．336，P〈0．05）。结论SDF-1在相对低氧状态下的视网膜中表达明显上调，因而可促进OIR的视网膜新生血管形成。Background The development of retinopathy of prematurity （ROP） is associated with many regulatory cytokines related to neovascularization;however,the retinal expression and regulated mechanism of stromal cell-derived factor-1 （SDF-1） in mouse model of oxygen-induced retinopathy （OIR） remain uncertain. Objective This study was to investigate the expression of SDF-1 in retina of mouse model of OIR. Methods Forty 7-day-old C57BL/6J mice were divided into OIR group and control group. In OIR group,20 mice were exposed to 75% oxygen for 5 days and then to room air for 5 days. In control group,20 mice were raised in room air. The expression of SDF-1 in retina of mice was studied by immunochemistry and quantified by real time reverse transcriptase polymerase chain reaction （RT-PCR）. Results The positive immunohistochemical staining for SDF-1 was found mainly locating at the ganglion cell layer in 12-day-old mice of OIR group;the stronger positive immunohistochemical staining for SDF-1 was noted mainly locating at the ganglion cell layer, vascular endothelial cells of inner retina, neovascular endothelial cells in 17-day-old mice of OIR group; the delicate positive immunohistochemical staining for SDF-1 was both found mainly locating at the inner retina and being around the retinal vascular in 12-day-old mice of control group and 17-day-old mice of control group. The expression of SDF-1 mRNA in 17-day-old mice of OIR group was higher than that of 12-day-old mice of OIR group （t = 8. 072, P〈0.05 ） and 17-day-old mice of control group （ t = 10. 026, P〈0.05 ）, respectively. The expression of SDF-1 mRNA in 12-day-old mice of OIR group was lower than that of 12-day-old mice of control group （t =4. 336,P〈0. 05）. Conclusion SDF-I might improve the onset of retinal neovascularization of OIR.

关 键 词：基质细胞衍生因子-1 氧诱导视网膜病变 疾病模型/小鼠 视网膜新生血管 

分 类 号：R774.103[医药卫生—眼科]