鼻咽癌细胞中ezrin基因增强子区的定位分析  

Localization of ezrin Enhancer in Nasopharyngeal Carcinoma Cells

作　　者：高书颖[1] 代延朋[1] 尹美珺[1] 龙霞[1] 于洁[1] 

机构地区：[1]深圳北京大学香港科技大学医学中心北京大学深圳医院中心实验室,深圳518036

出　　处：《中国细胞生物学学报》2011年第7期777-783,共7页Chinese Journal of Cell Biology

基　　金：中国博士后科学基金项14(No.20090450100;No.201003361);广东省自由科学基金项目(No.9152800001000017;No.9151030002000008);深圳市卫生局;科技和信息局项目(No.JC200903180676A;No.200901012;No.200801001)资助项目~~

摘　　要：本研究采用嵌套缺失和荧光素酶检测技术对鼻咽癌CNE2细胞ezrin基因增强子区进行定位分析。实验结果显示,CNE2细胞中,ezrin基因-1541/-706具有转录激活和转录增强作用,存在转录正调控区和负调控区。对5个潜在转录调控区的进一步研究发现,ezrin基因-1297/-1186对ezrin启动子和SV40启动子具有显著的转录增强作用;其它4个区域对启动子不表现转录调控作用,或表现弱的转录增强作用。结果表明,ezrin基因-1297/-1186是具有增强子作用的关键转录调控区,它有可能与其它潜在转录调控区以共同或协同的方式调控ezrin基因转录。To localize human ezrin gene enhancer in nasopharyngeal carcinoma cells, a series of reporter gene expression vectors were constructed using nested-deletion method and then were detected via luciferase assay. In CNE2 cells, it was found that ezrin -1541/-706 possessed promoter and enhancer activity, and there were some positive and negative transcriptional regulatory regions within this -1541/-706 region. Further studies on 5 poten- tial transcriptional regulatory regions revealed that ezrin -1297/-1186 enhanced transcription of ezrin promoter and SV40 promoter significantly, other 4 regions showed absent regulation or weaker enhancement on promoters. These data suggested that the ezrin -1297/-1186, a key transcriptional regulatory region as an enhancer, probably regu- lated ezrin transcription in company with other potential regulatory regions.

关 键 词：鼻咽癌 EZRIN基因 增强子 转录调控 

分 类 号：R739.63[医药卫生—肿瘤]