雷帕霉素对人外周血T淋巴细胞上BTLA表达的影响  

The influence of rapamycin on BTLA expression of human peripheral blood T lymphocytes

作　　者：丁晨光[1] 田普训[1] 燕航[1] 薛武军[1] 葛冠群[1] 靳占奎[1] 李杨[1] 

机构地区：[1]西安交通大学医学院第一附属医院肾病中心肾移植科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2011年第7期751-753,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30872578;30753761);陕西省科技攻关项目基金资助(2008K13-04);西安市科技计划资助项目(SF08006-2);陕西省自然科学基金资助(SJ08C2012004C247);陕西13115重大科技攻关资助项目(S2009ZDKG138)

摘　　要：目的：探讨雷帕霉素（rapamycin,RPM）对人外周血T淋巴细胞上B、T淋巴细胞弱化子（BTLA）表达的影响,为其在器官移植和自身免疫性疾病中的应用提供实验依据。方法：采用Ficoll密度梯度离心法分离人外周血单核细胞（PBMCs）,然后利用免疫磁珠分离技术分离PBMCs中的T细胞。用刀豆蛋白A刺激、活化人外周血T细胞。用MTT比色法测定T细胞的增殖。用ELISA法测定细胞培养上清液中IL-2、IFN-γ的水平。用流式细胞术检测T细胞上BTLA的表达。结果：用不同浓度（10~1 000 mL）的RPM处理的T淋巴细胞上BTLA的表达无显著差别;而与未经RPM处理组比较差别显著（P〈0.01）。ELISA检测结果显示不同浓度的RPM与未处理组相比均能显著抑制IL-2和IFN-γ的分泌（P〈0.01）,并可以剂量依赖的方式显著抑制T淋巴细胞增殖（P〈0.01）。结论：RPM对BTLA的表达影响较小,而对T淋巴细胞增殖和IL-2、IFN-γ的分泌有较强的抑制作用,提示RPM适宜用于器官移植排斥反应和自身免疫性疾病的治疗。AIM：To explore the effect of rapamycin（RPM） on the expression of B and T lymphocyte attenuate（BTLA） on human peripheral blood T lymphocytes,providing a experimental basis for application of RPM to organ transplantation and autoimmune diseases.METHODS：Human peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation,and then the T cells were isolated from PBMCs with immunomagnetic beads.Concanavalin A（Con A） used to stimulate and activate the human peripheral blood T cells.The proliferation of T cells was detected by MTT colorimetry.The levels of IL-2 and IFN-γ in cell culture supernatant were detected by ELISA.The expression of BTLA on T cells was assayed by Flow cytometry.RESULTS：The expression of BTLA on T cells treated with various concentrations（10-1 000 ng/L） of RPM had no significant difference,while had significant difference（P0.01） by compared with RPM non-treatment group.ELISA detection manifested that compared with the untreated group different concentrations of RPM could significantly inhibit the IL-2 and IFN-γ secretion and significantly inhibited T lymphocyte proliferation（P0.01）.CONCLUSION：RPM has little effect on expression BTLA,but has stronger inhibition on lymphocyte proliferation and inflammatory cytokines secretion.Suggesting that RPM is suitable for the treated of organ transplant rejection and autoimmune diseases.

关 键 词：雷帕霉素 BTLA T细胞 排斥反应 自身免疫病 

分 类 号：R392.12[医药卫生—免疫学]