宫颈液基细胞中转录因子Brn-3a表达对宫颈病变的早期诊断价值  

Evaluation of Brn-3a immunocytochemistry of liquid-based ThinPrep cytology test in early diagnosis of cervical process

作　　者：郭晓东[1] 牛柯[2] 赵景民[1] 熊璐[1] 张昕[1] 赵雨来[1] 

机构地区：[1]解放军第302医院,北京100039 [2]解放军总医院第一附属医院妇产科,北京100048

出　　处：《细胞与分子免疫学杂志》2011年第7期801-802,804,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:探讨宫颈液基细胞中转录因子Brn-3a表达及其早期诊断宫颈病变的临床价值。方法:选择我院病理科有活检或手术切除标本病理诊断的液基细胞学检查(TCT)患者192例,并均行阴道镜下活组织检查。将入选病例剩余TCT标本进行Brn-3a蛋白免疫细胞化学染色,并与组织病理学结果进行对照。结果:TCT残留标本中Brn-3a蛋白表达的阳性率随宫颈病变严重程度加重而增高,且各级细胞学诊断间比较差异具有统计学意义(P<0.05)。单纯TCT的病理诊断符合率为SCC 96.8%(30/31),HSIL80.5%(33/41),LSIL76.9%(30/39),而TCT联合Brn-3a的病理诊断符合率为SCC 97.1%(30/31),HSIL78.6%(33/42),LSIL76.9%(30/39)。与单纯TCT的病理诊断符合率比较,TCT联合Brn-3a对HSIL、LSIL的病理诊断符合率明显高于单纯TCT(P<0.05)。结论:TCT联合Brn-3a检测对早期诊断宫颈病变具有一定的临床价值。AIM：To investigate the value of Brn-3a immunocytochemistry of liquid-based ThinPrep cytology test（TCT） in early diagnosis of cervical process.METHODS：Patients（192） were investigated in the present study.TCT was used in all the patients.Immunohistochemistry was performed to examine the expressions of Brn-3a.The pathological results were used as the gold standard to evaluate the results of TCT method and Brn-3a in combination with LCT.RESULTS：Compared with the pathological results,the accuracies of TCT method were 76.9%（30/39）,80.5%（33/41） and 96.8%（30/31）in LSIL,HSIL,SCC respectively.The accuracies of Brn-3a in combination with TCT were 76.9%（30/39）,78.6%（33/42） and 97.1%（30/31） in LSIL,HSIL and SCC respectively.The difference was significant in the diagnosis of LSIL and HSIL（P0.05）.CONCLUSION：The detection of Brn-3a is of important clinic value in early diagnosis of cervical process.

关 键 词：宫颈癌 液基细胞学 BRN-3A 

分 类 号：R737.33[医药卫生—肿瘤]