壳寡糖对大鼠肠缺血再灌注损伤的保护作用  

Protective Effects of Chitosanoligosacchariden on Intestinal Injury Induced by Intestinal Ischemia/reperfusion in Rats

作　　者：周春燕[1] 黄智铭[1] 杨庆[1] 吴金明[1] 吴建胜[1] 张启瑜[2] 

机构地区：[1]温州医学院第一附属医院消化内科,325000 [2]温州医学院第一附属医院肝胆外科,325000

出　　处：《医学研究杂志》2011年第7期91-94,共4页Journal of Medical Research

摘　　要：目的探讨壳寡糖对大鼠肠缺血再灌注(I/R)损伤肠黏膜屏障功能的保护作用及其可能的作用机制。方法采用夹闭肠系膜上动脉(SMA)的方法制作轻度和重度肠缺血再灌注损伤模型。将40只成年雄性SD大鼠随机分为5组,分别为假手术组、重度缺血再灌注组、轻度缺血再灌注组、壳寡糖对轻度及重度缺血再灌注干预组。再灌注后用Ch iu氏评分法观察肠黏膜的损伤情况,检测血清二胺氧化酶(DAO)与小肠组织髓过氧化物酶(MPO)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平以及小肠黏膜组织的SIgA水平。结果小肠缺血再灌注后,肠黏膜形态学损伤明显,血清中反应肠损伤的DAO以及肠组织匀浆中MDA及MPO含量明显升高,SOD活性明显降低,肠黏膜组织的SIgA水平明显下降,运用壳寡糖预处理的轻度及重度I/R组以上变化均较相同I/R时间模型组减轻。结论壳寡糖对大鼠轻度和重度肠缺血再灌注损伤后的肠黏膜屏障有保护作用,该保护作用可能与清除氧自由基、减少中性粒细胞聚集与活化以及增强大鼠肠黏膜免疫屏障功能有关。Objective To investigate the protective effects of chitosanoligosacchariden on intestinal injury induced by ischemia/ reperfusion（I/R） and the possible mechanism. Methods Intestine ischemiareperfudion modle induced by totally occludding the superior mesenteric artery（SMA） was set up. 40 male SD rats were randomly divided into 5 groups. Every group had 8 rats. They were sham group,modle of severe I/R injury, modle of mild I/R injury, and chitosanoligosaccharid intervention groupes. After reperfusion, Chiu＇s count was used to assess the changes in intestinal pathological morphology. SOD activity and MDA content in intestinal, DAO activity in serum and SIgA activity of intestinal mucous were observed. Results After ischemia/reperfusion,morphology injury of intestinal was obvi- ous. The contents of DAO in blood serum and the contents of MDA and MPO in intestinal homogenate were all increased obviously, and SOD decreased obviously. SIgA level in intestinal mucous was decreased obviously. Above changes in chitosanoligosacchariden interven- tion groups were obviously alleviated compared with those in model groups. Conclusion Chitosanoligosacchariden can protect the intesti- nal mucosal barrier of rats with gut injury from I/R damage,which may related to removing oxygen free radical,reducing accumulation and activation of neutrophilic granulocyte and enhancing intestinal immunity system.

关 键 词：缺血-再灌注损伤 壳寡糖 肠 

分 类 号：R574[医药卫生—消化系统]