Measurement and Study of Tyrosinase Activity in Potato  

马铃薯中酪氨酸酶活性测定及研究(英文)

作　　者：王宁芳[1] 

机构地区：[1]青海师范大学,青海西宁810008

出　　处：《Agricultural Science & Technology》2011年第6期799-800,804,共3页农业科学与技术（英文版）

摘　　要：[Objective] The paper was to study the activity of tyrosinase,so as to provide reference for preventing browning of potatoes as well as fruits and vegetables containing tyrosinase.[Method] With potato as raw material and Na2HPO4-HCl in buffer solution（pH=7.2）as system,spectrophotometer was used to measure the absorbance of potato extract at 480nm,the curve was established,and the activity of tyrosinase was obtained.[Result] The changes of data were little under the above conditions,and the stability of tyrosinase activity was high.[Conclusion] Using spectrophotometry to determine the tyrosinase activity in potato is simple with high accuracy.[目的]研究酪氨酸酶的活性,为防止马铃薯及其它含酪氨酸酶的水果和蔬菜等发生褐变提供参考。[方法]以马铃薯为原料,以pH=7.2的Na2HPO4-HCl缓冲溶液为体系,用分光光度计在480nm处测定马铃薯提取液的吸光度,建立曲线,从而得出酪氨酸酶的活性。[结果]在该条件下,所测数据波动小,酪氨酸酶的活性稳定性高。[结论]采用分光光度法测定马铃薯中酪氨酸酶活性,方法简便、准确度高。

关 键 词：TYROSINASE ABSORBANCE Kinetic curve ACTIVITY 

分 类 号：S532[农业科学—作物学]