太平洋牡蛎杂交三倍体与诱导三倍体的生长比较  

Growth Comparison of Induced and Mated Triploid Pacific Oysters

作　　者：孔静[1] 王昭萍[1] 于瑞海[1] 张跃环[1] 刘剑[1] 李晓瑜 李雅琳 郭希明 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]青岛崂东海珍品良种培育有限公司,山东青岛266105 [3]Haskin Shellfish Research Laboratory,Institute of Marine and Coastal Sciences,Rutgers University

出　　处：《中国海洋大学学报（自然科学版）》2011年第7期67-71,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30771622);国家高技术研究发展规划项目(2006AA10A401)资助

摘　　要：本文以中国太平洋牡蛎二倍体自交作为对照,比较了诱导三倍体群体及其杂交三倍体的早期表型性状。通过对各试验组卵径、卵裂率、D幼率、D形幼虫大小、生长、存活率和倍性等指标进行测定,进一步评估了2种诱导方法的有效性。结果表明:与诱导三倍体群体相比,杂交三倍体表现出明显优势,D幼率、D形幼虫大小均大于诱导三倍体群体,且差异显著(P<0.05);稚贝期壳长和壳高生长也显著快于诱导三倍体群体(P<0.05)。至附着,杂交三倍体的存活率很高,显著优于诱导三倍体群体。就三倍体率而言,杂交三倍体达到100%,而诱导三倍体群体仅为51.00%±11.79%。由上得知,杂交三倍体更适合应用于大规模生产。Performance of induced and hybridized triploid Pacific oysters（Crassostrea gigas） was studied and compared for traits such as fertilization and hatching success,survival,larval and juvenile growth.The results showed that hybridized triploids were superior over induced triploids,and the difference in hatching success and D-larval size was significant（P0.05）.Larval survival of the hybridized triploids was significantly higher than that of induced triploids.Juvenile shell height and shell length of hybridized triploids were larger than that of induced triploids,showing their superiority in growth.Flow cytometry analysis showed that the hybridized group contained 100% triploids,and the induced group had only（51.0±11.8）% triploids.It is evident that hybridized triploids are superior over triploids induced by chemical treatment.Our study shows that mating diploids and tetraploids is an ideal approach to triploid production and is extremely valuable for large-scale production of triploid oysters.

关 键 词：太平洋牡蛎 三倍体 二四倍体杂交 化学诱导 

分 类 号：Q321[生物学—遗传学] S944.41[农业科学—水产养殖]