RP-HPLC法测定油茶籽皂苷的含量  

Determination of camellia saponin content by RP-high performance liquid chromatography

作　　者：吴向东[1] 彭彤[1] 颜钫[1] 王丽[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064

出　　处：《海南医学院学报》2011年第8期1017-1020,共4页Journal of Hainan Medical University

基　　金：四川省科技厅科技支撑项目(2009FZ0059)~~

摘　　要：目的:建立RP-HPLC测定油茶籽中皂苷含量的方法。方法:色谱柱为waters spherisorb ODS柱(5μm,250mm×4.6mm),流动相为乙腈∶水(98∶2),检测波长234nm;流速0.8mL/min,柱温为25℃,理论板数不低于10 500。结果:线性方程为Y=38404 X-2285,相关系数r=0.9998,线性范围为1.39～6.96mg/mL,浓度与线性关系良好。试验精密度RSD值为0.56%。试验重现性RSD值为1.17%。试验的稳定性RSD值为1.86%,油茶皂苷溶液在24h内稳定。加样回收试验,平均回收率为99.89%,RSD值为0.88%。结论:该方法快捷、简便,重现性好,灵敏度高,可用于油茶籽中皂苷的含量测定。Objective. To establish a method for determination of camellia saponin content determi- nation in Camellia oleifera Abel seeds by RP-HPLC. Methods. Chromatographic column.waters spherisorb ODS column （5fire, 25 mm x 4.6 mm）, mobile phase-acetonitrile, water （98：2）, detected wavelength.. 234 nm, flow velocity.-0.8 mL/min, column temperature：25℃, theoretical plate number should be more than 10500. Results.. The calibration curve linear equation is Y = 38404X - 41.39, correlation coefficient r 0.9998, the calibration curve was linear range of 1.39 mg/mL 6. 96 mg/mL. Test precision RSD was 0.56%. Test reproducibility RSD was 1.17 % and stability RSD was 1.86 %. Camellia saponins so lution became stable within 24 h. The average recovery was 99.89% with RSD of 0.88 %0. Conclusions： This method is quick, convenient, reproducible with high sensitivity and can be used for the determinationof camellia saponin content inCamellia oleifera Abel seeds.

关 键 词：油茶籽 RP-HPLC 油茶皂苷 

分 类 号：R93[医药卫生—生药学] R945[医药卫生—药学]