软腐白菜根系土壤中香味类香味菌的分离与鉴定  

Isolation and Identification of a Myroides odoratus Strain in Rhizospheric Soil of Chinese Cabbage Soft Rot

作　　者：杜迅[1] 胡宜亮[1] 何蔚荭[1] 陈国参[1] 刘丽[1] 王亚南[1] 

机构地区：[1]河南省科学院生物研究所有限责任公司河南省微生物工程重点实验室,河南郑州450008

出　　处：《环境与健康杂志》2011年第7期611-613,共3页Journal of Environment and Health

基　　金：河南省重大科技攻关计划项目(082101310200)

摘　　要：目的鉴定软腐白菜根系土壤中分离的条件致病菌——香味类香味菌。方法采用选择性培养基,从软腐白菜根系土壤中分离出15株产生芳香气味的细菌,通过对菌株进行形态学观察、生理生化试验以及16S rRNA基因序列测定、比对分析和系统发育树构建,对该类群菌株进行初步分类鉴定。结果软腐白菜根系土壤中分离获得的15株产生芳香气味的细菌中,有一株菌其形态学特征及生理生化特性均与香味类香味菌Myroides odoratus ATCC4651T(M58777)一致,16S rRNA部分基因序列与香味类香味菌基因序列同源性达99.99%。初步鉴定该菌为香味类香味菌。结论采用选择性培养基,结合经典分类学方法和16S rRNA基因序列比对分析、构建系统发育树,可分离并鉴定软腐白菜根系土壤中的香味类香味菌。Objective To isolate and identify the conditioned pathogenic bacteria strain Myroides odoratus. Methods With selected medium, a conditioned pathogenic bacteria strain was isolated from the rhizospheric soil where the Chinese cabbages were planted. The isolated strain was identified according to its 16S rRNA gene sequence analysis as well as its morphological features, physiological and biochemical analysis. Its taxonomic status was confirmed by 16S rRNA gene sequence homologous analysis and phylogenetic tree. Results The standard strain exhibited the highest levels （99.99%） of similarity to the Myroides odoratus M58777,the results of several methods and morphological observation,this strain was finally identified as Myroides odoratus. Conclusion There is a conditioned pathogenic microorganism, Myroides odorotus, in the rhizospherie soil of Chinese cabbages.

关 键 词：土壤 16S RRNA 鉴定 香味类香味菌 

分 类 号：R117[医药卫生—公共卫生与预防医学]