兔下颌骨缺损修复中骨保护素及配体和环氧合酶-2的表达  

Expression of osteoprotegerin, osteoprotegerin ligand and cyclooxygenase-2 in the repairing process of mandibular defect in rabbit

作　　者：翁春辉[1] 赖晓宇[1] 詹春华[1] 戴丽冰[2] 钟志勇 

机构地区：[1]广州市红十字会医院口腔科,510220 [2]广州市红十字会医院创伤外科研究所,510220 [3]广东省医学动物实验中心,广州市528248

出　　处：《实用医学杂志》2011年第15期2712-2714,共3页The Journal of Practical Medicine

摘　　要：目的:探讨骨保护素(Osteoprotegerin,OPG)、骨保护素配体(Osteoprotegerin ligand,OPGL)和环氧合酶-2(Cyclooxygenase-2,COX-2)在下颌骨缺损修复中的作用机制。方法:健康雄性新西兰大白兔48只,随机分为对照组(24只)和实验组(24只),建立下颌骨缺损模型,分别于建模后3、14、28、56d处死相同例数动物(n=6),然后收集下颌骨缺损处的骨组织块。采用免疫组织化学、RT-PCR技术检测OPG、OPGL、COX-2蛋白及mRNA表达变化。结果:兔下颌骨缺损修复过程中,OPG、OPGL、COX-2蛋白和mRNA在不同时间点均有不同程度高表达(P<0.05),但各指标峰值出现的时间点不同(P<0.01)。OPG,OPGL,COX-2峰值分别出现在建模后3、28、14d。结论:兔下颌骨缺损修复中OPG、OPGL、COX-2蛋白及mRNA表达水平不同程度高表达,提示其可能参与兔下颌骨缺损修复的过程。Objective To explore the repair mechanism of osteoprotegerin （OPG）, osteoprotegerin ligand （OPGL） and cyclooxygenase-2（COX-2） in mandibular defect. Methods 48 healthy male New Zealand rabbits were randomly divided into the control group （24） and the experimental group （24）. On 3 d, 14 d, 28 d, 56 d after the mandibular defect modele was established, 6 rabbits were killed separately. At the same time, the bone in the zone of mandibular defect was collected. The expression of protein and mRNA including OPG, OPGL and COX-2 were tested by means of immunohistoehemical technique and RT-PCR. Results The protein and mRNA of OPG, OPGL and COX-2 were highly expressed to varying degrees at different time points （P 〈 0.05）. However, the maximum peak of three indexes presented at different time points separately （P 〈 0.01 ）. OPG, OPGL, COX-2 presented their each maximum peak on 3 d, 28 d, 14 d respectively after establishing model. Conclusion The protein and mRNA of OPG, OPGL and COX-2 were highly expressed to varying degrees at different time points, which can be concluded that they may oarticioate in the repairing process of the mandibular defect.

关 键 词：骨保护素 骨保护素配体 环氧合酶-2 下颌骨缺损 

分 类 号：R782[医药卫生—口腔医学]