羊膜对激光角膜切除术后TGF-β_1表达调控的初步研究  

The Preliminary Study of Amniotic Membrane on TGF-β_1 Expression after Photorefractive Keratectomy

作　　者：钟一声[1] 周颖明[1] 王康孙[1] 

机构地区：[1]上海第二医科大学瑞金医院眼科中心,上海200025

出　　处：《眼科学报》1999年第4期215-217,235,共4页Eye Science

基　　金：国家自然科学基金资助(批准号:39940007)

摘　　要：目的:探讨羊膜对激光角膜光学切除术(Photorefractive keratectomy PRK)后角膜组织TGF-β_1表达的影响。方法:对6只新西兰白兔双眼行PRK,术后1眼立即行角膜表面羊膜移植术,另眼作为对照。术后4周用原位杂交方法检测角膜上皮和基质TGF-β_1mRNA的表达。结果:PRK后4周对照组角膜上皮和基质有较浓密的TGF-β_1mRNA表达,羊膜移植组角膜上皮和基质亦有TGF-β_1mRNA表达,其表达程度较对照组轻(P<0.05)。结论:羊膜移植能降低PRK后角膜组织TGF-β_1mRNA的表达。眼科学报1999;15:215-217。Objective :To investigate the effect of amniotic membrane on TGF-β1 expression after photorefractive keratectomy (PRK). Methods: PRK was performed bilaterally on 6 New Zealand white rabbits. One eye was randomly tranplanted with preserved human amniotic membrane, and the other eye served as the control. The expression of TGF-β1 mRNA was detected with in situ hybridization at the 4th weeks after PRK. Results: TGF-β1mRNA expression was present in the corneal epithelium and stroma at the 4th weeks after PRK. The cornea in amniotic membrane transplanted group had also TGF-@1 mRNA expression, and the expression extent was less than that in control group (P <0. 05). Conclusion: Amniotic membrane can reduce the expression of TGF-β1 mRNA in cornea after PRK. Eye Science 1999; 15: 215 - 217.

关 键 词：羊膜移植 激光角膜切除术 TGF-Β1 角膜伤口愈合 

分 类 号：R779.63[医药卫生—眼科] R772.205[医药卫生—临床医学]