姜黄素固体脂质纳米粒单用或与顺铂联用对人卵巢癌SKOV3细胞增殖的抑制作用研究  

Inhibitory Effect of Curcumin-loaded Solid Lipid Nanoparticles Alone or Combined with DDP on the Growth of Human Ovarian Cancer Cell Line SKOV3

作　　者：邓舒婷[1,2] 甘霖[1] 周琦[1,2] 徐建业[3] 李少林[2] 

机构地区：[1]重庆市肿瘤研究所妇瘤科,重庆市400030 [2]重庆医科大学放射医学教研室,重庆市400016 [3]重庆市肿瘤研究所临床检验中心,重庆市400030

出　　处：《中国药房》2011年第29期2729-2731,共3页China Pharmacy

摘　　要：目的:研究姜黄素固体脂质纳米粒(Cur-SLN)单用或与顺铂(DDP)联用对人卵巢癌SKOV3细胞增殖的抑制作用。方法:单用试验,分组为空白对照组、Cur组、Cur-SLN组(均以Cur计,终浓度为10、20、40、60、80μmo·lL-1);联用试验,分组为空白对照组、DDP组、DDP+Cur组及DDP+Cur-SLN组,各组DDP终浓度为1、2、3、4、5μmo·lL-1,Cur终浓度均为10μmo·lL-1,检测上述各组分别对SKOV3细胞作用24、48、72h后细胞的生长抑制率。另设立空白对照组、DDP组、Cur组、Cur-SLN组、DDP+Cur组及DDP+Cur-SLN组(DDP终浓度均为2.5μmo·lL-1,Cur终浓度均为10μmo·lL-1),检测各组对SKOV3细胞作用24h后细胞凋亡率及细胞周期调控因子Cyclin E、CDK2的表达。结果:相同浓度下,与Cur组比较,Cur-SLN组作用不同时间后细胞生长抑制率均明显升高(P<0.05);与DDP组比较,DDP+Cur组和DDP+Cur-SLN组细胞生长抑制率均明显升高(P<0.05),且DDP+Cur-SLN组明显高于DDP+Cur组(P<0.05);与空白对照组比较,5个药物组细胞阻滞主要发生在G2期,细胞凋亡率和Cyclin E、CDK2表达均明显升高(P<0.05或P<0.01),且DDP+Cur-SLN组明显高于其他组(P<0.05)。结论:Cur-SLN对人卵巢癌SKOV3细胞有明显的生长抑制及促凋亡作用,且与DDP联用有协同效果。OBJECTIVE： To investigate the effect of Curcumin-loaded solid lipid nanoparticles （Cur-SLN） alone or combined with DDP on the growth of human ovarian cancer SKOV3 cells. METHODS： Single drug test groups were divided into blank con- trol group, Cur group and Cur-SLN group （the concentration of Cur were 10, 20, 40, 60, 80 μmol. L-1）. The drug combination test groups were divided into blank control group, DDP group, DDP＋Cur group and DDP＋Cur-SLN group （the concentration of DDP were 1, 2, 3, 4, 5 μmol.L -1, that of Cur was 10 μmol.L -1）. The inhibition rate of cell growth in above groups after 24 h, 48 h and 72 h were determined. Another group was divided into blank control group, DDP group, Cur group, Cur-SLN group, DDP＋Cur group and DDP＋Cur-SLN group（the concentration of DDP was 2.5 μmol.L -1, that of Cur was 10 μmol.L-1）. The apoptosis rate of SKOV3 cell after 24 h and the expression of cell cycle regulation factor Cyclin E, CDK2 of above groups were determined. RESULTS： With same concentration, the inhibition rate of SKOV3 cell growth in Cur-SLN group after different treatment time increased significantly, compared with Cur group （P〈0.05）. Compared with DDP group, the inhibition rate of SKOV3 cell growth in DDP＋Cur group and DDP＋Cur-SLN group increased significantly（P〈0.05）, that of DDP＋Cur-SLN group was signifi- cantly higher than DDP＋CUr group （P〈0.05）. Compared with blank control group, the other 5 groups could more significantly cause G2 phase arrest; the apoptosis rate and the expression of Cyclin E, CDK2 were increased significantly（P〈0.05 or P〈0.01）, and those of DDP＋Cur-SLN group were more significantly higher than other groups（P〈0.05）. CONCLUSIONS： Cur-SLN can significantly inhibit the proliferation of human ovarian cancer SKOV3 cells and induce cell apoptosis, and shows synergistic effects when combined with DDP.

关 键 词：姜黄素 固体脂质纳米粒 卵巢癌SKOV3细胞 顺铂 单用 联用 

分 类 号：R737.31[医药卫生—肿瘤]