固相萃取结合气相色谱法测定茶叶中三氯杀螨醇和拟除虫菊酯类农药残留  

Determination of dicofol and pyrethroid pesticide residues in tea by GC-SPE

作　　者：李晓晶[1] 于鸿[1] 贺小平[1] 黄聪[1] 甘平胜[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510440

出　　处：《中国食品卫生杂志》2011年第4期326-329,共4页Chinese Journal of Food Hygiene

摘　　要：目的建立茶叶中三氯杀螨醇和7种拟除虫菊酯类农药的检测方法。方法以丙酮作为提取溶剂,提取液经石墨碳黑/氨基(Carbon/NH2)固相萃取小柱净化后,用DB-5毛细管柱分离,采用气相色谱-电子捕获检测器(GC-ECD)进行测定。结果三氯杀螨醇和拟除虫菊酯类农药在20～800μg/L范围内线性良好,相关系数(r)均在0.998以上,最低检出限为0.23～1.38μg/kg,样品的加标回收率在88.5%～106.4%之间,相对标准偏差(RSD)在2.5%～10.7%之间。结论本方法净化效果好、灵敏度高、重现性好,可用于茶叶样品中三氯杀螨醇和拟除虫菊酯类农药残留量的检测。Objective To establish a method of determining dicofol and seven pyrethroid pesticide residues in tea.Methods Pesticide residues were extracted from tea samples by using acetone.Extracts were cleaned up by Carbon/NH2 SPE column.Pesticides were separated by DB-5 capillary column and determined by gas chromatography equipped with electron capture detector（GC-ECD）.Results The linear range of pesticides was from 20 to 800 μg/L with a good correlation coefficient（r0.998）.The minimum detection limit was 0.23-1.38 μg/kg.The recovery was in the range of 88.5%-106.4% with a RSD in the range of 2.5%-10.7%.Conclusion The purification effect of this method was good,and the detection was high in sensitivity and good at repeatability.The method could be applied to determine dicofol and pyrethroid pesticide residues in routine analysis of tea.

关 键 词：茶叶 三氯杀螨醇 拟除虫菊酯 气相色谱法-电子捕获检测器 农药残留 

分 类 号：TS272.7[农业科学—茶叶生产加工]