嘧啶醇对马铃薯种质试管苗保存遗传稳定性的影响  

Effects of ancymidol on long-term storage and genetic stability of potato plantlets in vitro

作　　者：柳寒[1] 谢婷婷[1] 徐君[1] 柳俊[1] 

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室/国家蔬菜改良中心华中分中心,武汉430070

出　　处：《华中农业大学学报》2011年第4期398-403,共6页Journal of Huazhong Agricultural University

基　　金：国家现代农业产业技术体系建设专项资金;湖北省自然科学基金项目(2009CDB254);中央高校基本科研业务费专项资金项目(2009QC019)

摘　　要：以3个不同基因型马铃薯品系Solanum chacosense（cha）、CE76和E3为试验材料,于基础培养基添加3种浓度（10、15、20μmol/L）的嘧啶醇,探讨不同浓度嘧啶醇对试管苗生长的影响;同时对添加嘧啶醇的培养基中生长的试管苗进行恢复培养后,选用RAPD和ISSR两种分子标记进行遗传稳定性分析,为嘧啶醇在马铃薯资源保存中的应用提供依据。结果表明：嘧啶醇可显著抑制3个马铃薯品系试管苗的生长（P〈0.01）,且不同浓度的嘧啶醇对不同马铃薯品系试管苗产生的抑制效率不同;恢复生长研究表明嘧啶醇不会导致试管苗的玻璃化或恢复生长缓慢等变异;遗传稳定性研究显示使用嘧啶醇保存的不同品系试管苗在DNA水平上均有极少量变异（平均变异率为0.48%-2.95%）产生,且不同品系变异率有一定差异。不同品系马铃薯试管苗保存应选用各自适合的嘧啶醇浓度。Ancymidol was investigated as a medium supplement for long-term storage of potato plantlets in vitro.Different concentrations of ancymidol（0,10,15,and 20 μmol/L） were evaluated in growth media based on MS medium with 80 g/L sucrose.The cultures of three genotype Solanum chacosense（cha）,CE76 and E3 were preserved under a 16 h photoperiod at（17±1）℃.The results showed that ancymidol had an active effect on culture viability to prolong preservation in vitro.Different concentrations of ancymidol had diverse growth-inhibiting effects.After conserved in ancymidol media,potato plantlets did not show evident abnormal shapes like vitrification and flaccidity in the following subculture,which are very frequently observed under mannitol stress.Genetic stability of potato plantlets conserved in ancymidol media was analyzed with randomly amplified polymorphic DNA（RAPD） and inter-simple sequence repeat（ISSR） fingerprints.Ancymidol resulted in 0.48%-2.95% genetic variations in genomic DNA of different genotypes,which will motivate us to find out better concentrations of ancymidol supplied for long-term storage of different genotypes.

关 键 词：试管苗保存 嘧啶醇 生长抑制 遗传稳定性 马铃薯 

分 类 号：S532.024[农业科学—作物学]