参附注射液对大鼠脑缺血再灌注后海马CA1区Caspase-3表达的影响  

Effect of Shenfu injection on the expression of Caspase-3 in the CA1 area of the hippocampus zone of rats after cerebral ischemia-reperfusion

作　　者：于腊梅[1] 李乃选[2] 王桂华[1] 刁汇玲[1] 张慧[1] 

机构地区：[1]滨州医学院生理学教研室,烟台市264003 [2]滨州医学院附属医院介入医学科

出　　处：《滨州医学院学报》2011年第3期179-181,共3页Journal of Binzhou Medical University

基　　金：滨州医学院科技计划(BY2009KJ29)

摘　　要：目的研究参附注射液对大鼠局灶性脑缺血再灌注后海马CA1区半胱氨酸蛋白酶-3(Caspase-3)表达的影响。方法成年雄性SD大鼠30只,随机分为假手术组、模型组、参附注射液治疗组。采用线栓法制备大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)再灌注模型,应用HE染色检测海马CA1区组织病理学改变,免疫组织化学法检测海马CA1区神经细胞Caspase-3的表达。结果缺血组大鼠海马CA1区神经细胞Caspase-3的表达较假手术组显著增强(P<0.01),给予参附注射液处理后,Caspase-3的表达较缺血组减弱(P<0.01),差异均有统计学意义。结论参附注射液可降低大鼠局灶性脑缺血再灌注后海马CA1区Caspase-3的表达,可能与其脑保护作用有关。Objective To study the effect of Shenfu injection on the expression of the Caspase-3 in CA1 hippocampus zone of rats after cerebral ischemia-reperfusion.Methods The 30 male Sprague-Dawley rats were divided into 3 groups：sham operation group,cerebral ischemia group and Shenfu injection intervented group.The focal cerebral ischemia models of rats were established by the middle cerebral artery occlusion（MCAO）with Longa＇s methods.HE staining was used to detect histopathological changes in CA1 area of hippocampus.The expression of Caspase-3 was observed by immunohisto-chemistry.Results Compared with the sham operation group,the expression of Caspase-3 increased obviously in cerebral ischemia group（P〈0.01）.After giving Shenfu injection,this changes were weaken（P〈0.01）.Conclusion Shenfu injection can inhibit apoptosis through descending the expression of Caspase-3 in CA1 hippocampus zone of rats after cerebral ischemia-reperfusion.

关 键 词：参附注射液 脑缺血再灌注 半胱氨酸蛋白酶-3 凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]