柄袋沙蠋体壁和消化系统甲硫氨酸脑啡肽免疫组织化学定位  

Immunohistochemical Localization of Methionine-enkephalin in the Body Wall and Digestive System of Arenicola brasiliensis

作　　者：黄雯雯[1] 王宜艳[1] 管晓娟[1] 孙虎山[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《四川动物》2011年第4期502-505,F0003,共5页Sichuan Journal of Zoology

基　　金：山东自然科学基金项目(ZR2010CM017)

摘　　要：采用免疫组织化学SABC法,对甲硫氨酸脑啡肽(M-ENK)在柄袋沙蠋体壁及其附属物鳃、疣足和消化系统各器官中的分布进行了研究。结果表明,柄袋沙蠋体壁、鳃、疣足以及各消化器官的上皮细胞中均有不同程度的M-ENK阳性反应,且体壁、消化道的食管、肠、直肠中阳性反应多集中在上皮细胞的游离端;体壁肌肉中环肌层M-ENK阳性反应比纵肌层强;食道侧囊上皮细胞和结缔组织中也有M-ENK分布。M-ENK在柄袋沙蠋的体壁、鳃、疣足及消化系统各器官中均有分布,且分布密度不同,可能与各部位的功能有关。The methionine-enkephalin（M-ENK） in the body wall and its components gill,parapodia and digestive system of Arenicola brasiliensis was analyzed by the immunohistochemical strept avidin-biotin complex（SABC） staining.The results showed that the different degree of positive M-ENK immunoreaction was observed in the epithelium of body wall,gill,parapodia and each organ of the alimentary.Besides,the positive immunoreaction was centralized in the dissociative sides of the epithelium of the body wall,oesophagus,intestine,rectum.In muscle of the body wall,the degree of M-ENK immunoreaction in the circular muscle layer was stronger than in the longitudinal muscle layer.There was M-ENK in the epithelium and the connective tissue of the oesophageal pouch too.It can be concluded that M-ENK is abundantly distributed in the body wall,gill,parapodia and digestive system in A.brasiliensis,and that different densities of M-ENK in these organs may be related to their different functions.

关 键 词：柄袋沙蠋 甲硫氨酸脑啡肽 体壁 消化系统 免疫组织化学 

分 类 号：Q51[生物学—生物化学]