生长激素在人T细胞白血病病毒1型Tax蛋白阳性T细胞中的表达及其对细胞活化的影响  

The expression of growth hormone and it＇s effection of activation in the T lymphocytes expressing HTLV-1 Tax

作　　者：邓保国[1] 牛志国[1] 王侠[1] 吕壮伟[1] 宋向凤[1] 王辉[1] 

机构地区：[1]新乡医学院免疫学研究中心,453003

出　　处：《中华微生物学和免疫学杂志》2011年第6期498-501,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30670910）

摘　　要：目的探讨成人T细胞白血病病毒1型（adult T cell leukemiavirustypel，HTLV-1）Tax蛋白对T淋巴细胞自身反应性生长激素（growthhormone，GH）表达的影响。方法Westernblot检测Tax阳性细胞（Tax Pcell）和Tax阴性细胞（TaxNcell）中的GH蛋白表达情况；共聚焦显微镜检测GH在两种细胞内的定位情况；RT-PCR检测GHmRNA的表达；脂质体法将pGL2-GH-luc转染TaxN和TaxP，检测GH的转录活性；脂质体法将pNF-KB-luc、pAP-1-luc和pNFAT—luc分别与pcDNA3．0-GH共转染TaxP细胞，检测核转录因子活性。结果与TaxN细胞相比，在TaxP细胞GH的mRNA和蛋白表达明显增高（P〈0．05），pGL2-GH-luc转染细胞后荧光素酶表达明显升高，约6．37倍（P〈0．05）；过表达生长激素可上调TaxP细胞中NF-κB活性1．7倍（P〈0．05）。结论生长激素可能协同NF-κB参与成人T细胞白血病（adult T-cell leukemia，ATL）的发病。Objective To study the effects of the adult T cell leukemia virus type 1 （ HTLV-1 ） Tax protein on T lymphocytes self-reactive growth hormone （GH）. Methods The expression of the growth hormone protein was measured by Western blot in TaxP and TaxN cells, which expresses HTLV-1 Tax or no. The location of growth hormone in the TaxP and TaxN cells were detected by LSCM（ laser scanning confocal microscope）. The level of growth hormone mRNA in TaxP and TaxN cells was measured by RT-PCR. The pGL2-GH-luc was transfected in TaxN and TaxP cells by Tfx-50-mediated transfection to assay transcriptional activity. The pNF-KB-luc, pAP-l-luc and pNFAT-luc was transfected into TaxP cells with pcDNA3.0-GH by Tfx50 to test bioactivity of the nuclear transcription factors. Results The mRNA and protein expression of GH could be promoted significantly by Tax. Relative to the TaxN cells, the transcriptional activity of GH was significantly increased about 6.37 times in TaxP cells（P〈0.05 ）. Overexpression of GH can increase the activity of NF-κ about 1.7 times in TaxP cells （P〈0.05）. Conclusion GH maybe involve in adult T-cell leukemia（ATL） through activating NF-κB.

关 键 词：HTLV-1 TAX蛋白 T淋巴细胞 生长激素 NF-κB 

分 类 号：R733[医药卫生—肿瘤] R3[医药卫生—临床医学]