荧光光谱法研究山奈素与β-环糊精及其衍生物包合作用  

Clathration of Kaempferol and β-Cyclodextrin and Its Derivatives by Fluorescence Spectrometry

作　　者：王浩江[1] 朱媛[1] 刘静纯[1] 张莉[1] 卞伟[1] 

机构地区：[1]山西医科大学基础医学院,太原市新建南路56号030001

出　　处：《光谱实验室》2011年第4期1698-1701,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：山西医科大学青年基金(057268);山西大学学生创新项目(201231)

摘　　要：采用荧光光谱法研究了β-环糊精(β-CD)、甲基-β-环糊精(M-β-CD)、羟丙基-β-环糊精(HP-β-CD)、磺丁基-β-环糊精(SBEβ--CD)与山奈素的包合特性。在固定山奈素浓度和改变环糊精及其衍生物浓度的情况下,山奈素荧光发射波长的变化以及荧光强度的增强表明了包合物的形成,用荧光双倒数法计算了环糊精及其衍生物与山奈素的包合常数。4种环糊精与山奈素的包合能力最强分别处在不同的pH体系中,包合物的包合比均为1∶1。The inclusion properties of kaempferol with β-cyclodextrin（β-CD）,methyl-β-cyclodextrin（M-β-CD）,hydroxyproyl-β-cyclodextrin（HP-β-CD） and sulfobutyl-β-cyclodextrin（SBE-β-CD） were studied by fluorescence spectrometry.Under unchanged the concentration of kaempferol and changed the concentration of β-cyclodextrin and its derivatives,the fluorescence emission wavelength of kaempferol changed,then fluorescence intensity enhancing showed clathrate compound formed,and the formation constants of kaempferol-CD and kaempferol were determined by fluorescence Lineweaver-Burk plot.The results showed that the four kinds CD had the strongest inclusion capacity with kaempferol in different pH buffer solutions.The inclusion ratios for the four kinds CD with kaempferol were all 1∶1.

关 键 词：山奈素 Β-环糊精及其衍生物 荧光光谱法 包合作用 

分 类 号：O657.32[理学—分析化学]