雌激素对实验性自身免疫性脑脊髓炎小鼠治疗作用的观察  

The Therapeutical Effect of Estrogen on Experimental Autoimmune Encephalomyelitis in Mice

作　　者：胡晓[1] 黄伟琨[2] 朱加应[3] 万兴[4] 王建怡[5] 

机构地区：[1]贵州省人民医院神经内科,贵阳550002 [2]贵州省人民医院口腔科,贵阳550002 [3]贵州省人民医院急诊科,贵阳550002 [4]贵州省人民医院肿瘤科,贵阳550002 [5]贵州省人民医院小儿内科,贵阳550002

出　　处：《天津医药》2011年第7期618-621,675,共5页Tianjin Medical Journal

基　　金：贵州省科技计划课题(课题编号:黔科合sy字[2009]3054);贵州省优秀科技教育人才省长专项资金项目[项目编号:黔省专合字(2010)86号]

摘　　要：目的:探讨雌激素对实验性自身免疫性脑脊髓炎(EAE)小鼠中枢神经系统(CNS)髓鞘及轴突损伤的影响。方法:用MOG35-55多肽诱发建立40只EAE小鼠模型后随机分为2组,各20只,治疗组予雌激素治疗,对照组予同等量的生理盐水灌胃处理。比较2组EAE小鼠的临床症状评分和体质量变化。取各组EAE小鼠脑和脊髓,行罗克沙尔固蓝(LFB)-HE染色及Bielschowsky染色观察髓鞘及轴突损伤情况;实时荧光定量PCR及Westernblot检测各组EAE小鼠CNS中髓鞘碱性蛋白(MBP)及生长相关蛋白-43(GAP-43)的表达。结果:治疗组EAE小鼠较对照组临床症状评分、平均丧失的最大体质量降低;LFB-HE染色、Bielschowsky染色示治疗组较对照组脱髓鞘减轻,轴突损伤减轻,PCR及Westernblot结果显示治疗组MBP和GAP-43表达较对照组增加。结论:雌激素可通过减轻髓鞘损伤及促进轴突再生治疗EAE小鼠。Objective：To observe the effect of estrogen on myelin and axonal injury in the central nervous system（CNS） of experimental autoimmune encephalomyelitis（EAE） in mice.Methods：Forty mouse EAE models were induced with MOG35-55 peptide.Mice were divided into treatment group and control group randomly（n=20 for each group）.The treatment group was treated by estrogen,and control group was given the same dose of saline.The clinical symptom score and body weight change were compared between the two groups.The myelin and axonal injury of the brain and spinal cord were studied by Luxol fast blue（LFB）-HE and Bielschowsky staining in mice.The expressions of myelin basic protein（MBP） and growth associated proteins（GAP）-43 were examined by real-time quantitative PCR and Western blot.Results：The clinical symptom score and average maximum loss weight were lower in treatment group than those in control group.The staining of LFB-HE and Bielschowsky showed that myelin and axonal injury was decreased in treatment group compared with that of control group.The results of quantitative real-time PCR and Western blot showed that the levels of MBP and GAP-43 were increased in treatment group than that of control group.Conclusion：Estrogen could treat EAE in mice by decreasing myelin damage and promoting axonal regeneration.

关 键 词：脑脊髓炎 自身免疫性 实验性 雌激素类 髓鞘 轴突 多发性硬化 髓磷脂碱性蛋白质类 GAP-43蛋白 疾病模型 动物 

分 类 号：R744.3[医药卫生—神经病学与精神病学]