黄芪注射液对阿霉素中毒性心肌损伤小鼠心肌细胞线粒体膜电位及GSH的影响  

Effect of Huangqi Injection on Mitochondrial Membrane Potential(MMP) and GSH in ADR Mice

作　　者：梁丽英[1] 陈晶[1] 贺毅[1] 

机构地区：[1]广西中医学院第一附属医院分子生物医学实验室,南宁530023

出　　处：《天津医药》2011年第7期622-624,共3页Tianjin Medical Journal

基　　金：广西中医学院自然科学研究课题(项目编号:P2007032);广西中医学院第一附属医院青年科研基金(项目编号:GZYQJ2011011)

摘　　要：目的:观察黄芪注射液对阿霉素中毒性心肌损伤模型(ADR)小鼠心肌线粒体膜电位及还原型谷胱甘肽(GSH)的影响。方法:取昆明小鼠60只,随机分为黄芪注射液治疗组(大、中、小3个剂量组)、阿霉素模型组和正常对照组,建立ADR,实验结束24h后处死小鼠,测定心脏系数和心肌组织中GSH的含量;应用流式细胞仪分析技术检测小鼠心肌细胞线粒体膜电位。结果:黄芪注射液各剂量组心脏系数与阿霉素模型组比较均显著降低(P<0.01);黄芪注射液各剂量组分别与阿霉素模型组比较GSH含量均增高(P<0.01);黄芪注射液各剂量组间心脏系数和GSH比较差异无统计学意义(P>0.05)。线粒体膜电位黄芪注射液各剂量组较阿霉素模型组明显增高(P<0.05或P<0.01),黄芪注射液各剂量组与正常对照组比较差异也有统计学意义(P<0.05)。结论:黄芪注射液可通过减轻小鼠心脏肿大,提高心肌内GSH含量,稳定线粒体膜电位,抑制心肌细胞发生异常凋亡来实现其保护心肌细胞的作用。Objective：To observe the effect of Huangqi injection on the mitochondrial membrane potential（MMP） and reduced glutathione hormone（GSH） in mice.Methods：Sixty Kunming mice were randomly divided into five groups,normal control group,adriamycin（ADR） group and Huangqi injection group（high-dose,middle-dose and low-dose）.Mouse models of myocardial injury were established by toxicity of doxorubicin.The values of the coefficient of the heart and GSH were detected,and MMP was tested by flow cytometry.Results：Compared with ADR group,there was a lower value of the coefficient of the heart in Huangqi injection group（P 〈 0.01）.The level of GSH was higher in Huangqi injection group than that of ADR group（P 〈 0.01）.There were no significant differences between the high-dose,middle-dose and low-dose of Huangqi injection groups（P 〉 0.05）.The value of MMP was significantly higher in Huangqi injection group than that of ADR group（P 〈 0.01）,and there was a significant difference between Huangqi injection group and normal control group（P 〈 0.05）.Conclusion：Huangqi injection protects cardiac muscle cells by relieving heart tumefy,increasing the level of GSH,stability MMP and restraining the apoptosis of cardiac muscle cells.

关 键 词：黄芪 注射剂 多柔比星 线粒体 细胞膜 电位测定法 谷胱甘肽 小鼠 

分 类 号：R-332[医药卫生]