丹曲林对缺血再灌注大鼠心肌糖代谢酶活性的影响  

Effect of Dantrolene on Activity of Glycometabolic Enzymes in Rats with Myocardial Ischemia-Reperfusion

作　　者：王丽艳[1] 赵晓丽[1] 郭彦青[1] 曹颖[1] 于公元[1] 康英姿[1] 

机构地区：[1]天津医科大学生物化学教研室,300070

出　　处：《天津医药》2011年第7期631-633,共3页Tianjin Medical Journal

基　　金：天津医科大学基金资助项目(项目编号:2009XK10)

摘　　要：目的:研究丹曲林对缺血再灌注损伤的大鼠心肌细胞中糖酵解和异柠檬酸脱氢酶活性的影响。方法:采用健康雄性Wistar大鼠16只建立Langendorff离体心脏灌注模型,分为对照组和丹曲林预处理组,对照组全心缺血30min,再灌注60min;丹曲林预处理组加入5μmol/L丹曲林后,全心缺血30min,再灌注60min。用分光光度计法测定缺血再灌注后磷酸果糖激酶-1(PFK)、丙酮酸激酶(PK)、己糖激酶(HK)及异柠檬酸脱氢酶(IDH)的活性。结果:与对照组相比,丹曲林预处理组冠脉流量增加(P<0.01),PFK、PK、HK和IDH活性均降低(P<0.05或P<0.01)。结论:丹曲林预处理降低了糖代谢中关键酶的活性,对缺血再灌注心脏起到保护作用。Abtract Objective：To investigate the effect of dantrolene on the activity of glycometabolic enzymes in rats with myocardial ischemia-reperfusion.Methods：Sixteen healthy male Wistar rats were used in the Langendorff isolated heart perfusion system.The heart models were randomly divided into two groups：control group and dantrolene-treated group,with eight rats in each.The hearts in control group were subjected to 30 min of global ischemia and 60 min of retrograde reperfusion.The hearts in dantrolene-treated group were perfused for 20 min in the working mode,in the presence of 5 μmol/L dantrolene,and then were subjected to 30 min of global ischemia and 60 min of reperfusion.The activities of phosphofructokinase-1（PFK）,pyruvate kinase（PK）,hexokinase（HK） and isocitrate dehydrogenase（IDH） were determined by spectrophotometry.Results：The hemodynamic variables had no change except for increased coronary flow（P 〈 0.01）.Compared with the control group,the activities of PFK,PK,HK and IDH were significantly decreased in dantrolene-treated group（P 〈 0.05 or P 〈 0.01）.Conclusion：Dantrolene has a protective effect on myocardial ischemia-reperfusion by reducing the activities of glycolytic enzymes and isocitrate dehydrogenase.

关 键 词：丹曲林 心肌再灌注损伤 磷酸果糖激酶1 丙酮酸激酶 己糖激酶 异柠檬酸脱氢酶 大鼠 WISTAR 

分 类 号：R-332[医药卫生]