α-玉米赤霉醇对转APP基因小鼠的影响  

The effect of α-zearalanol(α-ZAL)on the APP transgenic mouse

作　　者：潘高峰[1] 毛善平[1] 张乾[1] 董慧敏[1] 严明敏[1] 付志平[1] 李燕[1] 

机构地区：[1]武汉大学人民医院神经内科,430060

出　　处：《卒中与神经疾病》2011年第3期142-144,183,共4页Stroke and Nervous Diseases

基　　金：国家985工程"认知与神经信息科学平台"资助项目(编号904273258);国家985工程"语言科学技术与当代社会建设跨学科创新平台"资助项目(编号985yk002)

摘　　要：目的研究α-玉米赤霉醇(-αZAL)对转APP基因构建的阿尔茨海默病(AD)小鼠模型的影响及其作用机制。方法采用转APP基因小鼠作为AD小鼠模型,-αZAL治疗组在小鼠9月龄后40d灌胃α-ZAL1.0mg/(kg.d)。Morris水迷宫实验观察-αZAL对AD小鼠认知功能的影响,测定血清和脑组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性和丙二醇(MDA)的含量。结果 AD模型组空间学习和记忆能力下降,血清和脑组织GSH-PX、SOD活力下降,MDA含量增多(P均<0.05);-αZAL治疗组空间学习和记忆能力升高,血清和脑组织GSH-PX、SOD活力升高,MDA含量减少,与AD模型组比较有显著差异(P<0.05)。结论 -αZAL可能通过抗氧化损伤保护机制来提高AD小鼠空间学习和记忆能力。Objective To explore the effect and the possible mechanisms of phytoestrogen α-zearalanol（α-ZAL）on the APP transgenic mouse model of Alzheimer＇s disease（AD）.Methods AD Mice model was established by transting APP gene.α-ZAL treatment group was treated with α-ZAL 1.0 mg/（kg·d） when they was nine months old for 40 days.The influence of α-ZAL on meory ability in AD mice was observed with morris test.The activities of glutathione peroxidase﹙GSH-PX﹚and superoxide dismutase﹙SOD﹚and the content of Malondialdehyde﹙MDA﹚in serum and brain tissue were measured.Results In AD model group,the there were decreased learning and memory abilities and decreased acticities of GSH-PX and SOD,and increased MDA content（P0.01）.In α-ZAL treatment groupstheere were learning and memory ability increased acticities of GSH-PX and SOD and decreaced MDA content There was significant difference between the two groups（P0.05）.Conclusions α-ZAL has a protective effect on cognitive function in AD Mice by improving antioxidation ability.

关 键 词：Α-玉米赤霉醇 抗氧化损伤 阿尔茨海默病 

分 类 号：R741[医药卫生—神经病学与精神病学]