局部应用唑来膦酸对拔牙创骨愈合影响的动物实验  

Effects of local application of zoledronic acid on bone healing of tooth extraction in rats

作　　者：马婷[1] 张文怡[2] 张健[1] 汪铭 崔彩雯[1] 

机构地区：[1]天津医科大学口腔医院口腔颌面外科,300040 [2]天津医科大学口腔医院修复科,300040 [3]南京中山医院口腔科

出　　处：《中华口腔医学杂志》2011年第7期403-405,共3页Chinese Journal of Stomatology

摘　　要：目的探讨局部应用唑来膦酸（zoledronic acid）对拔牙创愈合过程中I型胶原和血管内皮生长因子（vascular endothelial growth factor，VEGF）的影响。方法54只雄性Wistar大鼠按照随机数字法分为3组：唑来膦酸治疗组、明胶海绵组、空白对照组，每组18只，均拔除同一侧下颌第一磨牙。唑来膦酸治疗组于拔牙创内植入唑来膦酸-明胶海绵复合体；明胶海绵组植入明胶海绵；空白对照组不作处理。分别于术后1、2、4周取标本，应用免疫组化SP法观察I型胶原和VEGF的表达，并对染色灰度值进行统计学分析。结果术后1、2周，唑来膦酸治疗组I型胶原灰度值（60．00±1．81、63．47-4-3．02）均显著低于明胶海绵组（68．58±2．90、71．15±5．57）和空白对照组（69．164-9．63、72．50±4．10）（P〈0．05）；唑来膦酸治疗组和明胶海绵组4周I型胶原灰度值高于1、2周（P〈0．05）。术后1周，唑来膦酸治疗组VEGF灰度值（69．93±2．74）较明胶海绵组（60．86±4．79）及空白对照组（61．52±2．28）高（P〈0．05），唑来膦酸治疗组2周较1、4周低，差异有统计学意义（P〈0．05）。结论唑来膦酸局部用药可增加I型胶原的表达，但早期抑制VEGF的表达。Objective To investigate the effect of local application of zoledronic acid （ZA） on the expression of type I collagen and vascular endothelial growth factor （ VEGF ）. Methods Fifty-four male Wistar rats were divided into ZA group, gelatin sponge group, and blank control group after one-side tooth extraction. The expression of type I collagen and VEGF were detected with SP immunohistochemistry method 1, 2 and 4 weeks after operation. Results The gray value of type I collagen in ZA group （60. 00 ± 1.81, 63.47 ± 3.02 ）was lower than those in gelatin sponge group （68.58 ± 2. 90,71.15 ±5.57 ） and blank control group（69. 16 ±9. 63,72. 50 ±4. 10,P 〈0. 05）in the 1 and 2 week. In the ZA and gelatin sponge groups, the gray values of type I collagen were higher in the 4th week than in the 1st and 2nd week （ P 〈 0. 05 ）. The expression of VEGF was higher in ZA group （69.93 ± 2.74 ）than in gelatin sponge group （60. 86 ±4. 79 ）and blank control group （ 61.52 ± 2. 28 ） in the 1 st week （ P 〈 0. 05 ）. There was significant difference in VEGF expression between the 2nd week and 1 st and 4th week （P 〈0. 05 ）. Conclusions Local application of ZA could enhance the expression of type I collagen but inhibit the expression of VEGF in the early stage.

关 键 词：血管内皮细胞生长因子 I型胶原 唑来膦酸 

分 类 号：R782[医药卫生—口腔医学]