四种显像剂在NCI—H358肺癌裸鼠模型中显像效果和生物分布的比较  

Comparison of the biodistribution of four contrast agents in nude mice bearing NCI-H358 human lung cancer and evaluation of their value in diagnostic imaging

作　　者：张建阳[1] 冯珏[2] 李红梅[2] 李晓东[2] 贾红丽[2] 

机构地区：[1]保定市第一中心医院核医学科,071000 [2]河北医科大学第二医院核医学科,石家庄050000

出　　处：《中华肿瘤杂志》2011年第7期504-507,共4页Chinese Journal of Oncology

摘　　要：目的比较^18F-脱氧葡萄糖（^18F-FDG）、^99Tc^m-甲氧荩异丁基异腈（^99Tc^m-MIBI）^99Tc^m-氮-二（N-2基N-2氧基二硫代氧基甲酸盐）[^99Tc^m-N（NOET））]和^99Tc^m-RGD-4CK娃像剂任NCI—H358肺癌裸鼠中的摄取，评价4种硅像剂对低代谢肺胆瘤的诊断价值。方法建立人非小细胞肺癌NCIH358细胞裸鼠移植瘤模型20只，随机分为4组，分别经尾静脉注射^18F-FDG、^99Tc^m-MIBI、^99Tc^m-N（NOErr），和^99Tc^m-RGD-4CK移像剂，进行显像和生物分布研究。结果SPECT全身平面显像显示，^18F-FDG组倚瘤小鼠胸部放射性核素明妙浓聚，与肿瘤组织部分重叠，以敛肿瘤轮廓显示不清晰；^99Tc^m-MIBI组荷瘤小鼠右前肢肿瘤处隐约显影，轮廓不清；^99Tc^m-N（NOET）2组和^99Tc^m-RGD-4CK组荷瘤小鼠右前肢肿瘤部位硅影均清晰。^18F-FDG组、^99Tc^m-MIB组。^99Tc^m-N（NOET）：组和^99Tc^m-RGD-4CK组肺癌裸鼠模型显像的肿瘤勺肌肉放射性比值（T／NT）分别为1．85±0．07、0．99±0．16、2．78±2．26和3．40±0．27，生物分布的T／NT值分别为2．32±0．14、0．44±0．08、2．59±0．53和3．43±0．36，其^99Tc^m-RGD-4CK组的rf／NT值明显高于其他组（均P〈0．01）。结论^99Tc^m-RGD-4CK对人非小细胞肺癌NCI—H358细胞移植瘤的湿像效果好于^18F—FDG，在诊断低代谢的非小细胞肺癌时，^99Tc^m-RGD--4CK妊像町能比^18F—FDG显像更具优势。Objective To compare the uptake of four contrast agents： ^99Tc^m-RGD-4CK, ^99Tc^m-N （NOET）2, ^99Tc^m-MIBI and LSF-FDC in Bal B/c nude mice hearing human non-small cell lung cancer NCI- H358 and evaluate their diagnostic value in low-metabolic lung cancer. Methods ttuman bronehioloalveolar carcinoma NCI-H358 cells were subcutaneously inoculated in Bal B/c nude mice to establish mouse models bearing human lung cancer. Twenty tumor-bearing nude mice were given injection of the four contrast agent, respectively, 5 mice in each group. SPECT imaging and biodistribution of the 4 tracers in the tumor-bearing nude mice were performed. The ratios of tumor to non-tumor （T/NT） of the tracers were compared. Results The results from semi-quantification of the planar image and assessment of biodistribution showed that tumor to eontralateral muscle activity ratios （T/NT） of the four tracers had statistically significant difference between each two of the four tracer groups of tumor-bearing mice （ P 〈 0. 001 ） , with a highest value of T/NT ratio in the ^99Tc^m-RGD-4CK group. Conclusions NCI-H358 tumors show a higher uptake of ^99Tc^m-RGD-4CK than ISF-FDG. It suggests that when diagnosing a well- differentiated lung cancer such as bronchioloalveolar carcinoma, the contrast agent ^99Tc^m-RGD-dCK may be more sensitive than ISF-FDG, and it may become a promising contrast agent in tumor imaging diagnosis.

关 键 词：癌 非小细胞肺 NCI—H358细胞 ^18F-脱氧葡萄糖 ^99TC^M-甲氧基异丁基异腈 ^99Tc^m-氮-二(N-2基N-2氧基二硫代氧基甲酸盐) ^99Tc^m-RGD-4CK 

分 类 号：R734.2[医药卫生—肿瘤]