非小细胞肺癌表皮生长因子受体基因拷贝数与核苷酸切除修复交叉互补基因1和乳腺癌易感基因1的表达及其相关性分析  

EGFR gene copy number, ERCC1 and BRCA1 protein expression and their relationship in non- small cell lung cancer

作　　者：邓秋华[1] 邱源[1] 莫明聪[2] 张鑫[1] 熊信国[1] 江梅[1] 何萍[2] 温丹萍[1] 郑贵星[3] 何建行[1] 

机构地区：[1]广州医学院第一附属医院 广州市呼吸疾病研究所胸外科,510120 [2]广州医学院第一附属医院 广州市呼吸疾病研究所病理科,510120 [3]广州医学院第一附属医院 广州市呼吸疾病研究所输血科,510120

出　　处：《中华肿瘤杂志》2011年第7期508-512,共5页Chinese Journal of Oncology

摘　　要：腰目的探讨表皮生长因子受体（EGFR）基凶拷贝数与铂类耐药相关基因核苷酸切除修复交叉交互补基因1（ERCCl）和乳腺癌易感基因1（BRCA1）在非小细胞肺癌（NSCLC）中的表达情况，以及三者之间的相关性。方法用荧光原位杂交（FISH）技术检测EGFR基因的扩增情况，应用免疫组化SP法检测132例NSCLC患者标本ERCC1和BRCA1蛋白的表达，并进一步分析EGFR基因拷贝数、ERCC1和BRCA1的蛋白表达与患者临床病理特征的关系。结果EGFR基因FISH阳性率为24．1％（40／166）。女性的扩增阳性率高于男性（31．9％和18．6％，P=0．048）；无吸烟史的患者扩增阳性率明显高于有吸烟史的患者（32．8％和16．7％，P=0．045）；基冈拷贝数增加与患者的年龄、临床分期、病理类型及有无淋巴结或远处转移均无关。ERCCI蛋白表达阳性率为45．5％（60／132），在不同的病理类型分布比较，差异有统计学意义（P=0．046），与患者的性别、年龄、临床分期、有尢淋巴结或远处转移及吸烟状况均无关。BRCA1蛋白表达阳性率为35．1％（46／131），与患者的性别、年龄、临床分期、病理类型、淋巴结或远处转移及吸烟状况无关。ERCC1与BRCA1的蛋白表达呈中等相关（r=0．449，P〈0．001），与EGFR扩增无相关性（P=0．785）；BRCA1表达与EGFR基因拷贝数状态无相关性（P=0．143）。结论在肺癌的个体化治疗中，检测EGFR基因拷贝数、ERCC1和BRCA1的表达对靶向治疗和铂类药物化疗的选择具有重要的临床指导意义。Objective To evaluate the expression of epidermal growth factor receptor （EGFR） gcne copy number and the expression of ERCC1 and BRCA1 proteins in patients with non-small-cell lung cancer （NSCLC） and the correlation between them. Methods The status of EGFR gene copy number was determined by in situ hybridization （FISH）, and the expression of ERCC1 and BRCC1 proteins was examined by immunohistochemistry （IHC）. The relationship of EGFR gene copy number with the expression of ERCCI and BRCA1 and the clinical pathologic features were analyzed. Results FISH-positive EGFR expression was identified in 40 of 166 samples （24. 1% ）. More FISH-positive EGFR in the female than male patients （31.9% vs. 18.6% , P =0. 048） , and non-smoker than smoker （32.8% vs. 16.7% , P = 0. 045）. FISH-positive EGFR was not associated with age, pathological type, clinical stage and metestasis status （ P 〉 0.05）. The expression of ERCC1 protein was identified in 60 of 132 samples （45.5%）. The expression of ERCC1 protein varied significantly in tumors of different pathological types （P = 0. 046 ） , but not associated with age, gender, clinical stage, metestatic status and smoking status （P 〉 0.05）. The expression of BRCA1 protein was identified in 46 of 131 samples （35.1%）. The expression of BRCA1 was not associated with age gender, pathological type, clinical stage, metestatic ststus and smoking status （P 〉0.05）. There was a moderate correlation between the expressions of ERCC1 and BRCA1 （r = 0.449, P 〈 0.001 ）, but EGFR gene copy number was not correlated with the expression of ERCC1 or BRCA1 protein. Conclusions FISH-positive EGFR expression is associated with gender and smoking status, but not correlated with the expression of ERCC1 and BRCA1 proteins. There is a moderate correlation between the expressions of ERCCI and BRCA1.

关 键 词：癌 非小细胞肺 表皮生长因子受体 基因拷贝数 核苷酸切除修复交叉互补 基因1 乳腺癌易感基因1 

分 类 号：R734.2[医药卫生—肿瘤]