抗痫制剂对癫痫模型小鼠学习记忆变化的影响  

Effects of an anti-epileptic compound on learning and memory ability of epileptic mice

作　　者：王玢[1] 迟华基[2] 袁方曙[3] 

机构地区：[1]齐鲁师范学院生物系生物工程教研室,济南250013 [2]山东中医药大学基础医学院,济南250355 [3]山东大学医学院寄生虫学教研室,济南250012

出　　处：《山东大学学报（医学版）》2011年第7期53-56,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨抗痫制剂对戊四氮致痫小鼠学习、记忆力改变的影响。方法健康成年雄性小鼠60只,随机分为空白对照组,模型对照组,抗痫制剂大剂量组、中剂量组、小剂量组和丙戊酸钠组,每组10只。抗痫制剂大、中、小剂量组小鼠分别以抗痫制剂14.8 mg/g、7.4 mg/g和3.7 mg/g灌胃,每天1次,连续7 d;复制戊四氮诱导急性癫痫模型;水迷宫测定抗痫制剂对致痫小鼠学习和记忆力的影响。结果与空白对照组相比,模型对照组小鼠逃避潜伏期延长,行程出错率增加(P<0.05);与模型对照组和丙戊酸钠组相比,抗痫制剂大、中、小剂量组小鼠逃避潜伏期缩短、行程出错率减少(P<0.05)。与模型对照组相比,丙戊酸钠组小鼠逃避潜伏期、行程出错率均值虽增加,但差异无统计学意义(P>0.05)。结论模型对照组和丙戊酸钠组小鼠学习、记忆力降低;抗痫制剂能缩短模型小鼠水迷宫逃避潜伏期,减少行程出错率,明显改善致痫小鼠学习记忆能力。Objective To investigate the effects of an anti-epileptic compound on learning-memory ability of epileptic mice induced by pentetrazole.Methods Sixty healthy mature male mice were randomly divided into 6 groups（n=10 in each group）： the control group,the model group,three groups of the anti-epileptic compound at large,medium and small doses,and the sodium valproate group.Three groups of the anti-epileptic compound were intragastrically adminstered with doses of 14.8 mg/g,7.4 mg/g or 3.7 mg/g,and the treatment lasted for 7 days.The seizure model was established and water maze tests were conducted to evaluate the rats′ learning-memory ability.Results Compared to the control group,the escape latency extended and the ratio of mistakes increased in the model group（P〈0.05）;Compared with the model group and sodium valproate group,the escape latency shortened and the ratio of mistakes significantly decreased in all three anti-epileptic compound groups（P〈0.05）.In comparison with the model group,the escape latency and the ratio of mistakes made by mice increased in the sodium valproate group（P〉0.05）.Conclusion Learning and memory ability was reduced in the model group and the sodium valproate group.The anti-epileptic compound can shorten the escape latency and decrease the ratio of mistakes.It can obviously improve the spatial learning memory ability in epileptic mice induced by pentetrazole at different doses.

关 键 词：癫痫 模型 动物 抗痫制剂 学习和记忆 水迷宫 逃避潜伏期 行程出错率 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]