肾细胞癌血管增生与肝素酶、环氧化酶-2的关系  

Relationship of renal cell carcinoma angiogenesis with heparanase and cyclooxygenase-2

作　　者：田素建[1] 程丽[2] 徐忠华[1] 任巨超[1] 李大伟[1] 刘海南[1] 

机构地区：[1]山东大学齐鲁医院泌尿外科,济南250012 [2]山东中医药大学附属医院针灸科,济南250011

出　　处：《山东大学学报（医学版）》2011年第7期135-139,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术发展计划资助项目(032050166)

摘　　要：目的观察肾细胞癌(RCC)中肿瘤细胞生长、转移及肿瘤血管增生与环氧化酶2(Cox-2)、乙酰肝素酶(Hpa)的关系。方法采用免疫组化链霉亲和素-生物素-酶复合物法(SABC)法检测60例RCC组织标本和30例癌旁肾组织标本中的Cox-2、Hpa、CD34表达,分析COX-2、Hpa表达和肿瘤血管增生的关系,及微血管密度(MVD)与RCC组织学分级、临床分期和预后的相关性。结果 60例RCC组织标本中,Cox-2阳性率为71.7%,Hpa阳性率为63.3%,与癌旁肾组织比较差异有统计学意义(χ2=24.308,χ2=20.089,P均<0.05);Cox-2表达与RCC肿块大小差异有统计学意义(χ2=4.34,P<0.05);Hpa表达与肿块大小无统计学意义,但与患者淋巴结转移有密切的相关性(χ2=12.72,P<0.05);Cox-2、Hpa阳性和阴性表达组织中MVD值分别是125.74±35.68和138.56±40.74、70.87±23.64和80.88±21.52,差异有统计学意义(P均<0.05);MVD与RCC组织学分级、临床分期和预后密切相关(P<0.05)。结论 Cox-2高表达与肿块大小有相关性;Cox-2和Hpa可促进血管增生;MVD与患者预后密切相关。Objective To study relationships of tumor growth,metastasis and angiogenesis with protein expressions of cyclooxygenase-2（Cox-2） and heparanase（Hpa） in renal cell carcinoma（RCC）.Methods Immunohistochemical staining with StreptAvidin-Biotin Complex（SABC） was employed to determine expressions of Cox-2,Hpa and CD34 in 60 RCC tissues and 30 tumor-adjacent renal tissues.Cox-2 and Hpa expressions and microvessel density（MVD） in RCC tissues and tumor-adjacent renal tissues were analyzed and compared.Relations of MVD expression with clinicopathological parameters and prognosis were analyzed.Results Among 60 RCC tissues,positive rates of Cox-2 and Hpa expressions were 71.7% and 63.3%,respectively,and both of them were significantly higher than those in tumor-adjacent renal tissues（χ2=24.308,χ2=20.089,P〈0.05）.Increased expression of Cox-2 in RCC was associated with tumor size（χ2=4.34,P〈0.05）.Expression of Hpa was not related to tumor size,while it was significantly related to lymphatic metastasis（χ2=12.72,P〈0.05）.MVD were 125.74±35.68 and 70.87±23.64 in RCC with Cox-2-positive and negative expressions,and 138.56±40.74 and 80.88±21.52 in RCC with Hpa-positive and negative expressions,and there were significant differences among them（P=0.001）.MVD was related to histological grade,clinical stage and prognosis of RCC（P〈0.05）.Conclusion Enhanced expression of Cox-2 is associated with tumor size.Cox-2 and Hpa can promote angiogenesis.MVD is related to prognosis.

关 键 词：癌 肾细胞 环氧化酶-2 乙酰肝素酶 微血管密度 

分 类 号：R737.11[医药卫生—肿瘤]