低频重复经颅磁刺激预处理对氯化锂-匹鲁卡品致痫大鼠痫性发作及B细胞淋巴瘤／白血病基因-2、自杀因子和半胱氨酸天冬氨酸蛋白酶-3蛋白表达的影响  

The effect of low-frequency transcranial magnetic stimulation pretreatment on seizures, the expression of B cell lymphoma/leukemia gene-2, Fas protein and Caspase-3 protein in the hippocampal CA1 region in rats with epilepsy induced by lithium chloride -pilocarpin

作　　者：柯莎[1] 赵红宁[1] 张军强[1] 王晓明[2] 赵晓琼[2] 黄慧[2] 胡建秀[2] 

机构地区：[1]四川南充川北医学院附属医院神经内科,南充637000 [2]四川南充川北医学院附属医院神经疾病研究所,南充637000

出　　处：《中华物理医学与康复杂志》2011年第7期488-493,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：四川省青年基金资助项目（04ZQ026-010）

摘　　要：目的探讨低频重复经颅磁刺激（rTMS）预处理的抗痫作用及其与抗海马细胞凋亡的相关性。方法将健康成年Wistar大鼠30只分为rTMS预处理组、假刺激组及空白对照组，每组10只。rTMS预处理组行低频rTMS（0．5Hz、75％阈强度、20次／串、5串／d）预处理，假刺激组予以相同次数、声音相似的“假性”刺激，连续7d后，制作氯化锂-匹罗卡品诱导癫痫持续状态模型。观察各组大鼠痫性发作潜伏期及痫性发作程度，以免疫组织化学法观察各组大鼠海马CA1区B细胞淋巴瘤／白血病基因-2（Bcl-2）、自杀因子（Fas）及半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）蛋白的平均阳性反应细胞数及阳性反应细胞平均光密度。结果rTMS预处理组与假刺激组相比，痫性发作潜伏期显著延长、发作程度减轻（P〈0．01），各时点Bcl-2蛋白表达增加，Fas及Caspase-3蛋白表达减少。结论低频rTMS预处理具有抗痫作用，其机制可能与其调节海马区Bcl-2、Fas及Caspase-3蛋白表达水平，从而保护神经元有关。Objective To study the possible mechanisms by which repetitive transcranial magnetic stimulation （rTMS） pretreatment antagonizes seizures induced by lithium chloride-pilocarpine and any correlation with anti- apoptosis in hippocampal CA1 neurons. Methods Thirty rats were randomly divided into a control group, a sham stimulation group and an rTMS pretreatment group. The rTMS pretreatment group was pretreated on 7 consecutive days with low-frequency rTMS （0.5 Hz, 75% of threshold intensity, 20 times/bundle, and 5 bundles/d） , while the sham-stimulation group was sham-stimulated with a similar sound. Lithium chloride-pilocarpine （LPC） was used to induce a model epileptic state. Epileptic stroke latency and severity were recorded; neuronal morphology was observed using hematoxylin and eosin （HE） staining; mean positive-reactive cell number and mean optical density and absorbance of B cell lymphoma/leukemia gene-2 （Bcl-2） were recorded, and Fas and Caspase-3 protein in the hipp-ocampal CA1 region were observed with immunohistochemistry. Results Compared with the sham stimulation group, epileptic latency in the rTMS pretreatment group was significantly longer. Seizures in the rTMS pretreatment group were less severe, and a number of degenerated neurons were observed to be apoptotic. Bcl-2 protein expression increased at each time point, but Fas and Caspase-3 protein expression decreased. Conclusions rTMS pretreat- ment has an anti-epilepsy effect. The possible neuronal protection might be produced by regulating the expression of Bcl-2, Fas and Caspase-3 protein in the hippocampus.

关 键 词：低频重复经颅磁刺激 癫痫 B细胞淋巴瘤/白血病基因-2蛋白 自杀因子蛋白 半胱氨酸天冬氨酸蛋白酶-3蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学]