蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系MRP1表达的影响  

Effect of Agkihpin-an Arginine Esterase from Venom on Expression of MRP1 in CNE-2 Cells

作　　者：许淑茹[1] 马军[1] 袁志刚[1] 黄勇奇[2] 苏上贵[2] 胡启平[1] 

机构地区：[1]广西医科大学细胞生物学与遗传学教研室,南宁530021 [2]广西医科大学生化与分子生物学教研室,南宁530021

出　　处：《肿瘤防治研究》2011年第7期731-735,共5页Cancer Research on Prevention and Treatment

基　　金：广西青年基金资助项目(桂科青0728059);广西大型仪器协作共用网资助项目(700-2008-113)

摘　　要：目的探讨蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系中多药耐药相关蛋白MRP1表达的影响,并试图阐明Agkihpin抑制CNE-2细胞的机制。方法用不同浓度的Agkihpin处理细胞72h后,应用免疫细胞化学、Western blot、RT-PCR法检测MRP1在CNE-2细胞中的表达。结果不同浓度Agkihpin作用CNE-2细胞72h后MRP1表达均降低,并呈现出一定的浓度依赖效应,显示Agkihpin可显著下调CNE-2细胞中MRP1表达。各加药组与不加Agkihpin组比较,差异具有统计学意义(P<0.05)。结论在人低分化鼻咽癌CNE-2细胞系中,Agkihpin能抑制MRP1的表达,并且随浓度的增大抑制作用增加,这可能是Agkihpin能降低鼻咽癌细胞活力的原因之一;Agkihpin抑制MRP1的表达提示在一定程度上可提高肿瘤细胞对化疗药物的敏感度。Objective To explore the effect of Agkihpin on the expression of multidrug resistance associated protein 1（MRP1） in nasopharyngeal carcinoma CNE-2 cell line,and try to describe the mechanism of Agkihpin inhibiting CNE-2.Methods The cultured cells were treated with different concentrations of Agkihpin for 72 h.The expression level of MRP1 in CNE-2 cell line was assayed by immunocytochemistry,Western blot and RT-PCR.Results Comparing with Agkihpin absent group,the MRP1 expression levels in CNE-2 cells were reduced significantly after the treatment of different concentrations of Agkihpin（P0.05）,in a concentration-dependent manner,which shows that Agkihpin could significantly down-regulate the expression of MRP1 in CNE-2 cells.Conclusion Agkihpin can inhibit the expression of MRP1 in CNE-2 cells,which is likely to be one of reasons for Agkihpin inhibiting the cellular vitality of nasopharyngeal carcinoma cell,and be likely to enhance the sensitivity of CNE-2 cells to chemotherapeutic drugs to some extent.

关 键 词：多药耐药相关蛋白1 精氨酸酯酶 鼻咽癌细胞 

分 类 号：R739.63[医药卫生—肿瘤]