N-乙酰-L-色氨酸具有对新生鼠脑缺血/缺氧损伤的保护作用  

Protective effect of N-acetyl-L-tryptophan in cerebral ischemia/hypoxia injury

作　　者：蒋吉英[1] 姜桂兰[2] 李磊[1] 赵金岭[1] 史才兴[1] 于树娜[1] 齐安东[1] 

机构地区：[1]潍坊医学院人体解剖学教研室,山东潍坊261042 [2]青岛市精神卫生中心,山东青岛266034

出　　处：《解剖学报》2011年第4期451-455,共5页Acta Anatomica Sinica

基　　金：山东省自然科学基金资助项目(ZR2010HM006);山东省"泰山学者"建设工程专项经费资助项目

摘　　要：目的探讨N-乙酰-L-色氨酸(L-NAT)对新生鼠脑缺血/缺氧损伤的保护作用。方法出生第7天的沙土鼠90只,随机分为正常对照组、缺血/缺氧组和L-NAT组。制备脑缺血/缺氧损伤模型,L-NAT组在造模前30min腹腔注射L-NAT(5mg/kg)。用Fluoro-Jade B(FJB)染色法观察细胞的死亡情况;用免疫荧光染色、Westernblotting观察活化的Caspase-3和Caspase-9在各组的表达。结果 L-NAT可使缺血/缺氧损伤后FJB阳性细胞数量减少,并抑制缺血/缺氧后Caspase-3和Caspase-9的活化。结论 L-NAT对新生鼠脑缺血/缺氧损伤动物模型具有保护作用。Objective To investigate the possible neuroprotective effects of N-acetyl-L-tryptophan （L-NAT） in cerebral ischemia/hypoxia injury. Methods The ischemia/hypoxia injury models were made in 90 pups of seven-days old gerbil. The death of neurons was evaluated by Fluoro-Jade B（FJB） staining and the activations of Caspase-9 and Caspase-3 were evaluated by immunofluorescent staining and Western blotting. Results Our results showed that L-NAT treatment decreased the number of FJB positive cells after middle cerebral artery occlusion in mice. And L-NAT also inhibited the expressions of Caspase-3 and Caspase-9. Conclusion Our work demonstrated that L-NAT had protective effect on cerebral ischemia/hypoxia injury.

关 键 词：N-乙酰-L-色氨酸 脑 缺血/缺氧损伤 免疫荧光 免疫印迹法 新生沙土鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]