骨髓间充质干细胞移植脊髓全横断模型大鼠运动和体感诱发电位的评价  

Motor and somatosensory evoked potential evaluation in rat spinal cord transection models following bone marrow mesenchymal stem cell transplantation

作　　者：李宽新[1,2] 李锋[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,湖北省武汉市430022 [2]河子大学医学院一附院骨一科,新疆维吾尔自治区石河子市832000

出　　处：《中国组织工程研究与临床康复》2011年第23期4262-4266,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:临床常用皮质运动诱发电位和皮质体感诱发电位来分别评价脊髓损伤后运动传导路和感觉传导路的损伤或修复情况。目的:以脊髓诱导电位监测骨髓间充质干细胞移植后急性脊髓完全性损伤大鼠下肢神经功能的变化。方法:选取健康Wistar大鼠50只,分成5组,即生理盐水组、骨髓间充质干细胞移植组、脑源性神经营养因子修饰组、神经营养素3+骨髓间充质干细胞移植组和假手术组。除假手术组外,其余各组均制作Allen’s脊髓完全性损伤动物模型,造模后各组均行相应治疗。治疗后4,8和12周行大鼠后肢运动功能评分,并于造模后24h,3,7,14d行运动和体感诱发电位检测。结果与结论:运动诱发电位检测结果提示,各治疗组的运动功能均有不同程度的恢复,与生理盐水组间差异均有显著性意义(P<0.05),大鼠后肢BBB评分也证实了各治疗组后肢运动功能明显优于生理盐水组(P<0.05)。提示经脑源性神经营养因子修饰的骨髓间充质干细胞可移植到脊髓损伤处,可改善大鼠的后肢运动,神经营养素3蛋白有可能提高骨髓间充质干细胞在体内的生存率,促进受损脊髓的轴突再生。BACKGROUND：In clinic,cortical motor evoked potentials（MEP） and somatosensory evoked potentials（SEP） are commonly used to evaluate the injury or repair of motor and sensory pathways following spinal cord injury. OBJECTIVE：To investigate the treatment of acute complete spinal cord injury in rats with the transplantation of bone marrow mesenchymal stem cells（BMSCs） and to monitor the change in the neurologic functions of the lower limbs using MEP and SEP. METHODS：Fifty Wistar rats were randomized into saline group,BMSCs group,brain-derived neurotrophic factor（BDNF） group,neurotrophin 3＋BMSCs group,sham-operation group. Rats in the former four groups were used to produce Allen＇s models of spinal cord injury before treatment. At 4,8 and 12 weeks after treatment,rat hind limb motor function score was evaluated,and MEP and SEP were measured 1,3,7,14 days after modeling. RESULTS AND CONCLUSION：Using the MEP,we found that the motor function of each treatment groups was recovered to some extent. There was a significant difference between treatment groups and saline group（P 0.05） . The BBB score of the hind limbs showed that the motor function of the hind limbs in treatment groups was superior to that in the saline group（P 0.05） . BMSCs modified by BDNF could be transplanted into the injured spinal cord to improve the motor function of rat lower limbs. NT-3 could improve the survival rates of BMSCs and axonal regeneration following spinal cord injury.

关 键 词：脊髓完全性损伤 骨髓间充质干细胞 细胞移植 脊髓诱发电位 BBB评分 

分 类 号：R394.2[医药卫生—医学遗传学]