儿童急性淋巴细胞白血病β-arrestin1表达升高的临床意义  

Up-regulation of β-arrestin1 in children with acute lymphoblastic leukemia and its clinical significance

作　　者：刘慧[1,2] 龙娟[1] 谭俊杰[1] 孙滨[1] 张鹏辉[1] 邹琳[1] 

机构地区：[1]重庆医科大学附属儿童医院临床分子医学中心,重庆400014 [2]重庆医科大学附属第一医院输血科,重庆400016

出　　处：《第二军医大学学报》2011年第7期701-705,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(90919013,30871103);重庆市自然科学基金(2008BB5072,2010BA5008);教育部“新世纪优秀人才支持计划”(NCET-2008)~~

摘　　要：目的探讨β-arrestin1在儿童急性淋巴细胞白血病(ALL)中的表达及临床意义。方法收集155例初发ALL患儿的骨髓样本(ALL组),分离单个核细胞,以51例非恶性血液疾病患者作为对照(Ctrl组),将ALL患者分成高危、中危和标危组,采用实时荧光定量RT-PCR法检测β-arrestin1 mRNA的表达,采用蛋白质印迹法和免疫荧光法检测β-arrestin1蛋白表达的变化。用Spearman统计分析β-arrestin1表达与ALL患者临床和生物学特点之间的相关性。结果 ALL组β-arrestin1 mRNA和蛋白表达均高于Ctrl组(P值均<0.01)。高危组和中危组β-arrestin1 mRNA表达高于标危组,且高危组β-arrestin1 mRNA表达高于中危组,差异具有统计学意义(P<0.05)。Spearman相关分析显示,β-arrestin1表达与外周血白细胞数(r=0.458,P=0.002)和危险分级(r=0.344,P=0.022)正相关。结论儿童ALL患者骨髓β-arrestin1表达水平与患者的危险分级密切相关,对ALL诊断、治疗策略和预后具有重要意义。Objective To explore the expression of β-arrestin1 in newly-diagnosed childhood with acute lymphoblastic leukemia（ALL） and its possible clinical significance.Methods A total of 155 newly-diagnosed childhood ALL patients were enrolled in this study.Fifty-one sex-and age-matched patients without malignant hematological diseases were used as controls.The bone marrow samples were collected and from which the mononuclear cells were isolated.The β-arrestin1 mRNA expression was detected by quantitative real-time RT-PCR,and β-arrestin1 protein expression was detected by Western blotting analysis and immunofluorescence assays.Spearman correlation coefficient was used to analyze the correlations of β-arrestin1 expression with the clinical and biological features ALL patients.Results The expression of β-arrestin1 mRNA and protein in ALL patients was significantly higher than that in the controls（P0.01）.β-arrestin1 mRNA expression in ALL patients of high risk（HR） group and medium risk（MR） group was significantly higher than that of the standard risk（SR） group,and that in HR group was significantly higher than that in MR group（P0.05）.Spearman correlation analysis showed that β-arrestin1 expression was positively correlated with WBC count（r=0.458,P=0.002） and risk classification（r=0.344,P=0.022） of ALL patients.Conclusion Bone marrow β-arrestin1 expression is closely correlated with the risk classfication of child ALL patients,which is of great significance for the diagnosis,therapy strategy and prognosis of ALL.

关 键 词：急性淋巴细胞白血病 β-arrestin1 单个核细胞 

分 类 号：R733.711[医药卫生—肿瘤]