姜黄素、佛波酯对人卵巢癌细胞株SKOV3细胞生长的影响  

Effects of Curcumin,12-Tetradecanoyl-Phorbol-13-Acetate on Cell Growth of Human Ovarian Cancer Cell Line SKOV3

作　　者：龚萍[1] 李红霞[1] 张素梅[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院,北京100038

出　　处：《实用妇产科杂志》2011年第7期522-525,共4页Journal of Practical Obstetrics and Gynecology

基　　金：国家"十一五"863计划子课题妇科肿瘤标志物筛查资助(编号:2006AA02A245)

摘　　要：目的:通过研究抑癌剂姜黄素(CCM)、促癌剂佛波酯(TPA)对体外培养的人卵巢癌细胞株SKOV3细胞增殖、细胞形态及细胞周期的影响,探讨肿瘤细胞SKOV3增殖抑制的效应机制。方法:采用MTT法测定CCM、TPA不同浓度及不同时间对人卵巢癌细胞株SKOV3增殖的抑制作用,计算细胞生长抑制率。光镜和电镜下观测细胞形态学及超微结构的改变。流式细胞仪检测细胞周期。结果:TPA具有抑制人卵巢癌细胞株SKOV3细胞增殖的作用,呈剂量和时间依赖性,细胞生长停滞于G1/S期。部分细胞出现凋亡形态学改变。TPA抑制人卵巢癌细胞株SKOV3细胞的增殖,呈剂量和时间依赖性。细胞周期发生G1→S期阻滞。结论:CCM、TPA通过抑制细胞增殖,细胞周期发生停滞,显著地抑制卵巢癌细胞SKOV3的体外生长。Objective：To study the effects of Curcumin（CCM）、12-tetradecanoyl-phorbol-13-acetate（TPA） on the cell proliferation,morphology and cell cycle of human ovarian cell line SKOV3,to explore the inhibition mechanism of the human ovarian cancer cell line SKOV3.Methods：Growth inhibition rates of SKOV3 cells were measured by MTT method.Morphological changes of cells were observed under fluorescent invert microscope and electromicroscope.Cell cycle was checked by flow cytometery（FCM）.Results：CCM can inhibit cell proliferation of SKOV3 in a dose and time dependent manner,growth were hindered at stage G1/S.Partial cells presented the characteristic morphological changes of apoptosis.TPA can inhibit cell proliferation of SKOV3 cell in a does and time dependent manner.Growth were arrested at stage G1→S.Conclusions：CCM and TPA can significantly inhibit the growth of SKOV3 in vitro by inhibiting the cell proliferation and arrest the cell cycle.

关 键 词：姜黄素 佛波酯 人卵巢癌细胞株 细胞增殖 

分 类 号：R737.31[医药卫生—肿瘤]