水网藻对铜绿微囊藻的化感作用及对氮磷去除能力研究  

Allelopathy of Hydrodictyon reticulatum on Microcystis aeruginosa and Its Removal Capacity on Nitrogen and Phosphorus

作　　者：赵坤[1] 傅海燕[2] 柴天[2] 张明真[1] 刘智峰[3] 陈秀娟[2] 侯明[1] 许鹏成[1] 

机构地区：[1]华北电力大学能源与环境研究院,北京102206 [2]厦门理工学院环境工程系,厦门361024 [3]湖南大学环境科学与工程学院,长沙410082

出　　处：《环境科学》2011年第8期2267-2272,共6页Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2006CB403307;2005CB724200);国家水体污染控制与治理科技重大专项(2009ZX07104-004);厦门市科技计划项目(3502Z20093040);福建省科技计划项目(2008F5061)

摘　　要：通过测定藻液D680和叶绿素a浓度,研究了水网藻种植水对铜绿微囊藻生长的影响,探讨了在分离培养和共生培养2种条件下水网藻对铜绿微囊藻的抑制作用,并考察了水网藻对氮磷的去除能力.结果表明,水网藻对铜绿微囊藻有较强的抑制作用,在水网藻种植水作用8 d后,铜绿微囊藻死亡率达92%;不同浓度的水网藻对铜绿微囊藻的抑制作用不同,分离培养条件下3 g/L水网藻的抑制作用最强,10 d内D680从0.1下降到0.004,共生培养条件下4 g/L水网藻的抑制作用最强,抑制率为96%;比较2种条件下藻细胞大规模死亡的时间发现,分离培养下水网藻的抑制作用强于共生培养.2种条件下培养液中氮、磷浓度都有大幅度地降低,表明水网藻对氮磷有较强的吸收能力;氮、磷浓度下降速率与水网藻浓度成正比,10 d内氮、磷浓度最大下降幅度分别为93.4 mg/L和4.58 mg/L.The effects of liquid culture after cultured with Hydrodictyon reticulatum on the growth of Microcystis aeruginosa were investigated by measuring the D680value and the chlorophyll-a content of M.aeruginosa.The inhibitory effects of H.reticulatum on M.aeruginosa were studied in both isolated culture and co-culture conditions.Nitrogen and phosphorus removal capacity of H.reticulatum was also tested.Results showed that H.reticulatum could inhibit the growth of M.aeruginosa obviously.After treated by the liquid culture after cultured with H.reticulatum for 8 days,the mortality rate of M.aeruginosa reached 92%.The inhibitory effects of H.reticulatum at different concentrations on M.aeruginosa were different.The strongest inhibitory effect occurred with 3 g/L H.reticulatum in the isolated culture as the D680value reduced from 0.1 to 0.004 in 10 days,and it occurred with 4 g/L H.reticulatum in the co-culture as the suppression ratio was 96%.Comparing the large-scale death time for cells under these two conditions,the inhibitory effects of H.reticulatum in the isolated culture were stronger than those in the co-culture.The concentrations of nitrogen and phosphorus decreased sharply under both conditions,which showed that H.reticulatum had removal capacity on nitrogen and phosphorus.The decrease speed of nitrogen and phosphorus concentrations positively correlated to the concentrations of H.reticulatum.The highest decrease of nitrogen and phosphorus were 93.4 mg/L and 4.58 mg/L in 10 days,respectively.

关 键 词：水网藻 铜绿微囊藻 化感作用 分离培养 共生培养 氮磷去除 

分 类 号：X171[环境科学与工程—环境科学] X52