微囊藻伪空胞gvpA、gvpC与其物理性状之间相关性分析  

Correlation Analysis Among Characters of Gas Vesicle in Microcystis Strains

作　　者：张永生[1] 孔繁翔[1] 于洋[1] 张民[1] 史小丽[1] 施丽梅[1] 

机构地区：[1]中国科学院南京地理与湖泊研究所中国科学院湖泊与环境国家重点实验室,南京210008

出　　处：《环境科学》2011年第8期2273-2278,共6页Environmental Science

基　　金：国家重点基础研究发展计划(973)项目(2008CB418000)

摘　　要：为了探究gvpA基因拷贝数和gvpC基因内保守重复序列的作用,利用3株微囊藻包括铜绿微囊藻FACHB910、FACHB930以及惠氏微囊藻FACHB929为材料;测量了伪空胞长度、伪空胞直径、相对气体含量、表观压强、临界压强和细胞膨压等性状,分析了gvpA基因拷贝数目、gvpC基因内保守重复序列与这些性状之间的相关性.结果表明,gvpA基因拷贝数目与伪空胞相对气体含量呈极显著线性相关,相关系数为0.999;gvpA基因拷贝数目与伪空胞直径呈极显著负相关,相关系数为-0.861;gvpC基因内保守重复序列与伪空胞直径呈极显著正相关,相关系数0.911,与伪空胞相对气体含量、表观压强、临界压强呈极显著负相关,相关系数分别为-0.851、-0.999、-0.928.故推测:同一藻种内,gvpA基因拷贝数是影响伪空胞相对气体含量的主要因素;伪空胞的直径不仅受到gvpC基因内保守重复序列数量影响,而且还可能受到gvpA基因拷贝数调控.In order to explore the roles of gvpA copies and repeated sequences of gvpC,three Microcystis strains including M.aeruginosa FACHB910,M.aeruginosa FACHB930 and M.wesenbergii FACHB929 were used in this research.The length and diameter of gas vesicle,relative gas vesicle volume,appear pressure values,critical pressure values,cell turgor values were measured,and the correlations among these characters,gvpA copies,and repeated sequences of gvpC were analyzed.The results indicate that there are a significant positive linear correlation between gvpA copies and relative gas vesicle volume（r=0.999）;gvpA copies are negatively correlated with diameter of gas vesicle（r =-0.861）.However,repeated sequences of gvpC have a significant positive correlation with diameter of gas vesicle（r=0.911）,and have significant negative correlations with relative gas vesicle volume,appear pressure values and critical pressure values,with the correlation coefficient of-0.851,-0.999,-0.928 respectively.So we presume that gvpA copies are probably the primary impact factor for relative gas vesicle volume.The diameter of gas vesicle is not only regulated by repeated sequences of gvpC,but also regulated by gvpA copies.

关 键 词：微囊藻 伪空胞 gvpA基因拷贝数 gvpC基因内保守重复序列 相关性分析 

分 类 号：X52[环境科学与工程—环境工程]