逆转录病毒介导的野生型p53基因转入对慢粒多药耐药细胞恶性表型的影响  

RETROVIRUS MEDIATED GENE TRANSFER OF WT p53 INTO K562/HHT CELL LINE REVERSES ITS MALIGNANT PHENOTYPE

作　　者：陈学良[1] 宋丽[1] 刘春生[1] 丛雅琴 裴玉丽[1] 王应玞 

机构地区：[1]山东医科大学

出　　处：《山东医科大学学报》1999年第4期319-321,324,共3页Acta Academiae Medicinae Shandong

基　　金：卫生部科研基金!资助课题(96-2-153)

摘　　要：以Ｋ５６２／ＨＨＴ 细胞为模型，采用重组逆转录病毒载体介导的基因转移技术，将人野生型ｐ５３ｃＤＮＡ转入该细胞系，研究外源性野生型ｐ５３ 基因在白血病多药耐药细胞表达的意义。结果显示，外源性野生型ｐ５３ 基因抑制细胞增殖，一般形态学观察见细胞明显向成熟型分化。流式细胞仪分析还显示ｐ５３ 基因诱导Ｋ５６２／ＨＨＴ细胞死亡且伴有细胞周期Ｇ１ 期的生长阻滞。结果表明，野生型ｐ５３To study the significane of expression of exogenous wild type p53 gene(WP53G) in leukemic multidrug resistance cells, human WP53G cDNA was transferred by a recombinant retroviral vector in to K562/HHT cells. The Results suggest that exogenous WP53G inhibit cell growth and induce cell differentiation. Flow cytometric analysis showed that exogenous WP53G induced K562/HHT cells to die, which was accompanied by growth arrest in G 1 phase of the cell cycle. The conclusion can be drawn that the malignant phenotype can be at least partially reversed by restoring WP53G expression.

关 键 词：p53基因 逆转录病毒载体 白血病 基因治疗 

分 类 号：R733.705[医药卫生—肿瘤]