大肠癌中AgNORs计量、PCNA、p53的表达与预后  

Expression of PCNA and p53 and AgNORs Quantity in Colorectal Cancer and Its Prognostic Significance

作　　者：李太原[1] 戴安邦[1] 

机构地区：[1]江西医学院一附院,南昌市330006

出　　处：《实用癌症杂志》1999年第4期266-268,共3页The Practical Journal of Cancer

摘　　要：采用嗜银染色、ＡＢＣ免疫组化染色技术分别检测了６０ 例大肠癌组织中的ＡｇＮＯＲｓ 颗粒数、ＡｇＮＯＲｓ 面积、ＰＣＮＡ及ｐ５３ 的表达量，结合临床Ｄｕｋｅｓ′分期，采用Ｃｏｘ 回归分析评估ＡｇＮＯＲｓ 颗粒数、ＡｇＮＯＲｓ 面积、ＰＣＮＡ、ｐ５３ 表达量及Ｄｕｋｅｓ′分期在大肠癌预后评估中的作用。建立预后判别方程为ｐｖ ＝ｅ（（０ ．７１０２３８Ｘｌ ＋ ０．９１８５Ｘ２ ＋ ０．５２７５１３Ｘ３ ＋ ０．４６３１５６Ｘ４），判别值Ｌｎ（ｐｖ）≥１２．０ 时判别为存活５ ａ 以下，反之判为存活５ ａ 以上，灵敏度为９０．３％ ，特异度为９３．１ ％ ，准确率为９１ ．６％ 。提示ＡｇＮＯＲｓ 计量、ＰＣＮＡ、ｐ５３ 的表达多少可用于辅助评估大肠癌患者的预后。The eumeration and the area of AgNORs in the tissues of 60 cases of colorectal cancer were examined by argyrophilic staining techique.The expression of PCNA and p53 in above tissues were detected by immunohistochemistry method.A multivariate stepwise Cox's proportional hazard model analysis was performed to evaluate the prognostic value of the number of AgNORs,the area of AgNORs,the expression of PCNA,p53 and the Dukes′ grading.The formula was showed as fellowing.pv=e((0.710238X l+0.9185X 2+0.527513X 3+0.463156X 4),when Ln(pv)≥12.0,the patient was infered that he(she) couldn't survive over 5 year.When Ln(pv)<12.0,the patient was infered that he(she) could survive over 5 year.The efficienty was 91.6%,the sencitivity was 90.3%,the specificity was 93.1%.These results indicated that the AgNORs quantity and the expression of PCNA and p53 might be helpful to evaluate the prognosis of the patient with colorectal cancer.

关 键 词：大肠癌 预后 蛋白质 AGNOR染色 PCNA P53 

分 类 号：R735.3[医药卫生—肿瘤]