非霍奇金淋巴瘤中Cyclin D1和p34^(cdc2)蛋白的检测  

Detection of CyclinD1 and p34^(cdc2) in Non hodgkin′s Lymphomas

作　　者：江庆萍[1] 张俊德[1] 郭琳琅[1] 肖莎[1] 

机构地区：[1]第一军医大学珠江医院,广州市510282

出　　处：《实用癌症杂志》1999年第4期284-286,共3页The Practical Journal of Cancer

基　　金：广东省自然科学基金

摘　　要：为了研究非霍奇金淋巴瘤（ＮＨＬｓ）中细胞周期调节因子ＣｙｃｌｉｎＤ１ 和ｐ３４ｃｄｃ２ 蛋白表达与肿瘤分化程度和免疫分型的关系，对４０ 例ＮＨＬｓ 进行ＣｙｃｌｉｎＤ１ 和ｐ３４ｃｄｃ２ 蛋白免疫组化（ＳＰ法）染色。在４０ 例ＮＨＬｓ 中，有１９ 例（４７．５ ％）ＣｙｃｌｉｎＤ１ 阳性表达，２３ 例（５７．５ ％）ｐ３４ｃｄｃ２ 阳性表达。１０ 例淋巴结良性病变中６ 例生发中心细胞ＣｙｃｌｉｎＤ１ 和ｐ３４ｃｄｃ２ 弱阳性表达。低分化ＮＨＬｓ 的ＣｙｃｌｉｎＤ１ 和ｐ３４ｃｄｃ２ 阳性率明显高于高分化ＮＨＬｓ（Ｐ＜ ０．０５），而同免疫分型无关（ Ｐ＞０ ．０５） 。２ 个细胞周期调节因子的表达具有高度一致性（Ｐ＜０ ．０５）。提示２ 个细胞周期调节因子参与ＮＨＬｓ 的发生发展过程，其阳性表达率及表达强度同ＮＨＬｓ 恶性程度有密切关系，而同免疫分型无明显联系。ＣｙｃｌｉｎＤ１ 和ｐ３４ｃｄｃ２ 蛋白在部分ＮＨＬｓ 中共同起作用，使Ｇ１ →Ｓ期和Ｇ２ →Ｍ 期２ 个细胞检查点控制减弱。To research the relationship between expression of CyclinD1 and p34 cdc2 and Non hodgkin′s lymphomas(NHLs),40 cases of NHLs and 10 cases of reactive hyperplasias of lymphonodes(RHs) were studied by SP immunohistochemiscal technique.In 40 NHLs,19 cases(47.5%) had the CyclinD1 positive expression and 23 cases(57.5%) had p34 cdc2 expression,whereas in 10 RHs,CylinD1 and p34 cdc2 expression were found very faintly in germinal centers of 6 cases.CyclinD1 and p34 cdc2 positive rates in poorly differenetiated NHLs were much higher than those in well differnetiated NHLs( P <0.05).The positive rates of two factors were not related immune type of NHLs( P >0.05).There were high consistency between CyclinD1 and p34 cdc2 expression( P < 0.05 ).The results suggested that CyclinD1 and p34 cdc2 play a certain role during the development of NHLs,and as malignant degree of NHLs increases,their positive rates and grades will be higher.In some NHLs,two factors together may week the control of G 1→S and G 2→M points.

关 键 词：淋巴瘤 细胞周期素 细胞 P34^CDC2 蛋白 

分 类 号：R733.4[医药卫生—肿瘤]