谷胱甘肽S转移酶π、P-糖蛋白在骨肉瘤的表达及意义  

Clinical Significance of Expression of the Glutathione S transferase π and P glycoprotein in Osteosarcoma

作　　者：陈雷[1] 林建华[1] 张声[1] 陈林莺[1] 

机构地区：[1]福建医科大学附属第一医院,福州350003

出　　处：《福建医科大学学报》1999年第4期376-380,共5页Journal of Fujian Medical University

摘　　要：目的　检测谷胱甘肽Ｓ转移酶（ＧＳＴ－π）、Ｐ－糖蛋白在骨肉瘤、骨软骨瘤、正常骨组织中的表达及其与骨肉瘤临床病理改变之间的关系。　方法　对５３例原发性骨肉瘤、１５ 例骨软骨瘤、１７ 例正常骨组织进行免疫组化染色。　结果　在骨肉瘤中ＧＳＴ－π、Ｐ－糖蛋白的表达明显高于正常骨组织和骨软骨瘤，骨肉瘤中Ｐ－糖蛋白的表达与术前有无化疗明显相关，在骨肉瘤、骨软骨瘤和正常骨组织中Ｐ－糖蛋白与ＧＳＴ－π共同表达率分别为５２．８％ ， １３．３％ 和０。　结论　（１）骨肉瘤患者化疗不敏感可能与肿瘤组织中自发性存在Ｐ－糖蛋白和ＧＳＴ－π过表达有关；（２）术前化疗能明显提高Ｐ－糖蛋白的表达，提示骨肉瘤患者预后差可能与自发性和获得性Ｐ－糖蛋白的过表达有关；（３）联合检测ＧＳＴ－π、Ｐ－糖蛋白可作为鉴别良恶性骨肿瘤的有益指标。Investigation of the expression of the glutathione S transferase π(GST π) and the P glycoprotein in normal bone tissues,exostosis and osteosarcoma specimens,and the relationship between this expression and clinical pathology of osteosarcoma \ Methods\ The expression of the GST π and the P glycoprotein were evaluated immunohistochemically in 17 normal bone tissues,15 exostosis and 53 osteosarcoma \ Results\ The expression of the GST π and the P glycoprotein in osteosarcoma is markedly higher than those in normal bone tissues and exostosis \ The patients with preoperative chemotherapy were associated with overexpression of the P glycoprotein.\ The coexpression rate of the P glycoprotein and the GST π were 52 8%, 13 3%, 0% in osteosarcoma,exostosis and normal bone tissue specimen respectively \ Conclusion\ The patients with insensitivity to chemotherapy may be related to the spontaneous existence of P glycoprotein and the overexpression of the GST π \ The expression of the P glycoprotein can be intensively increased following the prechemotherapy \ The low survival rate of the osteosarcomas may have correlated with the spontaneous or obtained overexpression of the P glycoprotein \ The combined determination of GST π and P glycoprotein may be a useful method in the differentiation of benign and malignant bone tumor \;

关 键 词：P-糖蛋白 骨肉瘤 骨肿瘤 GST-Π 

分 类 号：R738.1[医药卫生—肿瘤]