贫血家兔红细胞寿命测定与膜蛋白形态学研究  

LIFE SPAN DETERMINATION AND STUDY ON MORPHOLOGY OF RED BLOOD CELL MEMBRANE PROTEIN IN ANEMIC RABBITS

作　　者：张圣明[1] 高培福[1] 潘庆仲[1] 鲁崎唔[2] 李如江[1] 李锋杰[1] 芨惠芬 

机构地区：[1]潍坊医学院组织学与胚胎学教研室,潍坊261042 [2]中国科学院生物物理研究所

出　　处：《解剖学杂志》1999年第6期504-508,共5页Chinese Journal of Anatomy

基　　金：山东省教育委员会资助;北京中关村地区联合分析测试中心的资助

摘　　要：目的：探讨溶血性贫血（溶贫）和再生障碍性贫血（再障）发病过程中红细胞（ＲＢＣ）膜的构架蛋白与细胞寿命的关系。方法：制备杂种家兔贫血动物模型，用冷冻蚀刻电镜技术和形态计量法观察分析溶贫兔、再障兔和正常兔ＲＢＣ膜蛋白颗粒的形态、分布、数量和大小。并用^(５１)Ｃｒ标记技术检测ＲＢＣ寿命。结果：溶贫兔和再障兔ＲＢＣ质膜内片劈裂面和外片壁裂面均见蛋白颗粒呈缗钱状、丛簇状分布，数量较正常兔减少（Ｐ＜０．０１）；内片劈裂面的颗粒大小和间隙与正常ＲＢＣ亦有显著性差异（Ｐ＜０．０１）。溶贫组和再障组的ＲＢＣ寿命明显短于正常组，溶贫组最短。结论：ＲＢＣ的寿命和功能与其胰蛋白颗粒分布、数量和质量可能有直接的关系。To explore the relationship between the life span and the structural proteins of red blood cell(RBC) membrane in the rabbits suffering from hemolytic anemia(HA) and aplastic anemia(AA). Methods: The morphology and distribution of the erythrocyte membrane protein granules were observed by freeze fracture etching and transmission electron microscope in the healthy rabbits and the rabbits with HA, AA. The total number and the size of erythrocyte membrane protein granules were measured by morphometry. The 51Cr-RBC method was used to determine the erythrocyte life span of 20 rabbits in each group respectively. Results: The erythrocyte membrane protein granules with HA and AA rabbits were distributed on a string of beads, plexus and groups in protoplasmic face(PF), and extracellular face(EF). The granule numbers from HA and AA were considerably less than that from healthy rabbits (PRO. of). There was a highly significant difference to normal rabbits in the size and space of the PF(P<0. of). The life span of erythrocytes in HA and AA rabbits was shorter than the normal, but the lift span of erythrocytes in HA rabbits was the shortest. Conclusion: There was certain relationship in quantity, quality and distribution of RBC membrane proteins with the lfie span and function of erythrocytes.

关 键 词：膜蛋白 红细胞 溶血性贫血 贫血 测定 

分 类 号：R556[医药卫生—血液循环系统疾病]