小鼠不全性肠梗阻对Cajal间质细胞表型的影响  

Phenotype alterations of interstitial cells of Cajal in mice with partial intestinal obstruction

作　　者：郭新[1,2] 刘东海[1] 黄旭[1] 陆红丽[1] 吴贻松[1] 韩燕飞[1] 许文燮[1] 

机构地区：[1]上海交通大学医学院生理学教研室,上海市200240 [2]南通大学附属医院泌尿外科,江苏省南通市226001

出　　处：《世界华人消化杂志》2011年第18期1886-1891,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.10672103;No.31071011~~

摘　　要：目的:探讨小鼠小肠慢性不全性肠梗阻导致自发性节律性收缩运动、电活动变化与Cajal间质细胞(ICC)表型转化的关系.方法:通过外科手术方法在小鼠回肠套入硅胶管,建立小鼠不全性肠梗阻模型.利用苏木精-伊红(H&E)染色,研究小肠平滑肌层形态学改变;利用常规生理和电生理技术观察环行平滑肌肌条自发性节律性收缩和慢波变化规律;利用全层平滑肌免疫组织化学方法,观察ICC表型标志酪氨酸激酶受体(c-kit)表达的变化.结果:小鼠不全性肠梗阻模型形成后14d,梗阻近端肠管显著扩张、小肠平滑肌层明显增生肥厚;小肠平滑肌自发性节律性收缩节律紊乱、幅度不规则、频率变慢;平滑肌静息膜电位去极化、慢波幅度变小、频率变慢;ICC表型标志c-kit表达显著减弱、甚至消失.结论:小鼠不全性肠梗阻导致机械和电活动紊乱与ICC表型转化导致的ICC数量减少和网络破坏有关.该模型为进一步研究ICC表型改变的细胞/分子机理提供了一个良好的实验模型.AIM： To investigate changes in mechanic and electrical activities in intestinal smooth muscle and the phenotypes of interstitial cells of Cajal （ICC） in mice with partial intestinal obstruction.METHODS： A mouse model of partial mechanical ileal obstruction was induced by surgery. The histochemical technique was used to investigate morphological changes in the distended intestinal regions of model mice 14 d after surgical induction. Mechanic and electric activities were recorded in normal and distended intestinal circular muscle using conventional physiological and intracellular recording techniques. The expression of c-kit, an ICC phenotype marker, was examined by fluorescent immunohistochemistry. RESULTS： Fourteen days after surgical induction, there was an increase in intestinal diameter and hypertrophy of the tunica muscularis. Decreased frequency and altered rhythm of spontaneous contractions of intestinal smooth muscle were noted. The amplitude and frequency of slow waves and resting membrane potential decreased significantly. The expression of ICC was significantly down-regulated. CONCLUSION： The changes in spontaneous rhythmic contractions and the slow waves are associated with the phenotype alterations of ICC in mice with partial intestinal obstruction.

关 键 词：CAJAL间质细胞 酪氨酸激酶受体 慢波 不全性肠梗阻 

分 类 号：R574.2[医药卫生—消化系统] R-332[医药卫生—内科学]